T细胞受体γ引物组合检测蕈样肉芽肿基因重排的研究

Abstract

Abstract: CHEN Liu-qing, CHEN Jin-bo, DUAN Yi-qun, LI Dong-sheng, DONG Bi-lin, ZHANG Hong-mei, YU Xin. Department of Dermatology, Wuhan First Hospital, Wuhan 430022, China 【Abstract】 Objective To estimate the value of BIOMED-2 primers for the detection of T cell receptor γ （TCR-γ） gene rearrangements in different types of specimens from patients with mycosis fungoides （MF）. Methods Totally, 15 paraffin-embedded tissue specimens, 14 fresh tissue specimens and 18 whole blood specimens were obtained from 28 patients with MF, and subjected to DNA extraction. BIOMED-2 multiplex PCR tubes TCRγ （A+B） were used for the analysis of TCRγ gene rearrangements. Data were processed by SPSS 13.0 software, and statistical analysis was done by chi-square test and Fisher's exact probability test. Results TCRγ gene rearrangements were detected in 3 paraffin-embedded tissue specimens, 11 fresh tissue specimens and 12 blood specimens, with significant differences in the detection rate between the three samples （χ2 = 13.047, P < 0.01）. The fresh tissue samples showed a significantly higher detection rate than the paraffin-embedded tissue samples （χ2 = 12.523, P < 0.01）. The detection rate of TCRγ gene rearrangements was 3/6 in paraffin-embedded tissue samples collected in 2011, significantly higher than that in the other 9 paraffin-embedded tissue samples collected before 2011 （Fisher′s exact probability test, P = 0.044）, but similar to that in 14 fresh tissue specimens （12/14, Fisher′s exact probability test, P = 0.044）. Decreased detection rate of TCRγ gene rearrangements was observed in blood samples compared with fresh tissue specimens, but no statistical difference was observed between the two types of specimens （χ2 = 2.358, P > 0.05）. Conclusions BIOMED-2 multiplex PCR tubes TCRγ（A+B） are suitable for the detection of clonal rearrangements of TCRγ gene in different types of specimens, especially in fresh tissue specimens, from patients with MF. 【Key words】 Gene rearrangement, gamma-chain T-cell antigen receptor; Mycosis fungoides; Polymerase chain reaction

Key words: PCR

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Application of BIOMED-2 primers in the detection of T cell receptor γ gene rearrangements in patients with mycosis fungoides

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