Abstract: Objective To study the effects of heparinase antisense oligodeoxynucleotide(ASODI)on the expression of heparinase mRNA and protein in human malignant melanoma cell line A375.Methods Heparinase ASODN and its controlled nonsense oligodeoxynucleotide(N-ODN)were designed and synthesized.A375 cells were divided into three groups: ASODN group, transfected with 30 μmol/L of heparinase ASODN and lipofectin; N-ODN group, transfected with 30 μmol/L of heparinase N-ODN and lipofectin; blank control group.Heparinase mRNA and protein were detected by in situ hybridization and immunohistochemistry, respectively.Results At 24 h after the transfection, the expression of heparinase mRNA was significantly lower in the ASODN group than that in the N-ODN group and control group (1.250±0.500 vs 4.000±0.817, 1.250±0.500 vs 4.250±0.500, both P<0.01).The expression of heparinase protein was also significantly decreased in the ASODN group than that in the control and N-ODN group at 48 h after transfection(5.000±0.817 vs 1.500±0.577, 4.500±0.577 vs 1.500±0.577, both P<0.01).Conclusion Heparinase ASODN could down-regulate the expression of heparinase mRNA and protein in A375 cells.

Key words: Melanoma, Heparin lyase, Oligonuclcotidcs, antisense