Chinese Journal of Dermatology ›› 2007, Vol. 40 ›› Issue (3): 133-135.

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Molecular epidemiology of tetM gene in high-level tetracycline-resistant Neisseria gonorrhoeae isolates in Nanjing, 1999-2004

JIANG Fa-xing, Qi Mu-ge, DAI Xiu-qin, SUN Hou-hua, YE Shun-zhang, SU Xiao-hong   

  1. Institute of Dernaatology, Chinese Academy of Medical Sciences & Peking Medical College, Nanjing 210042, China
  • Received:2006-03-09 Online:2007-03-15 Published:2007-03-15

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Abstract: Objective To investigate the molecular epidemiology of tetM gene in Neisseria gonorrhoeae strains with high level resistance to tetracycline(TRNG)in Nanjing.Methods TRNG were detected by an agar dilution method.β-lactamase production was examined by paper acidometric testing.A single tube PCR was used to subtype tetM gene in TRNG.Results The isolation rate of TRNG increased year by year from 1999 to 2004, and a total of 136(16.92%)TRNG strains were isolated out of 804 Neisseria gohorrhoeae strains in Nanjing during this period.Of these TRNG, 69.85%(95/136)produced β-lactamase (PPNG/TR'VG).Subtyping of tetM gene showed that 135 TRNG were Dutch variant, while only one was American variant.Conclusion In Nanjing, Dutch variant is the predominant tetM determinant in TRNG, whereas American variant is the sporadic determinant.

Key words: Neisseria gonorrhoeae, Tetracycline resistance, tetM gene