Abstract: Objective To investigate the effects of keratin 17(K17) antisense oligonucleotide(ASODN) on psoriasis using a SCID-hu xenogeneic transplantation model.Methods Psoriatic Lesions were obtained from patients with plaque psoriasis and grafted onto the skin of SCID mice to develop the SLID-hu xenogeneic transplantation model.Totally,12 SCID mice were divided into three groups,and liposomal emulsions containing Kl7 ASODN,K17 SODN and vehicle alone were applied topically to grafts in the individual groups,respectively.The changes of grafts were observed macrographically as well as histopathogically with HE staining.The expression of K17 mRNA and protein was detected in the three groups by RT-PCR and immunohistochemistry,respectively.Results The grafts maintained all the characteristics of psoriasis after transplantation,and animal model of psoriasis was established successfully.The grabs' inflammation was recovered more rapidly,and histological score reduced significantly in ASODN group than those in SODN and control groups.Also,the expression of K17 mRNA and protein was decreased significandy in ASODN group than that in other two groups as shown by RT-PCR and immunohistochemistry.Conclusions KI7 ASODN could markedly relieve the pathological changes of psoriatic grafts in SCID-hu xenogeneic transplantation model,and is expected to be a new approach to the treatment of psoriasis.

Key words: Keratin, Oligonucleotides,antisense, Mice,SCID, Psoriasis, Models,animal