Abstract: Objective To explore the relationship between fluconazole resistance and expression of multidrug resistant protein genes,including CDR1,CDR2,MDRI.Methods The total RNA was extracted from fluconazole-resistant and-susceptible Candida albicans isolates,and cDNA was synthesized.The expression of CDR1,CDR2 and MDRl genes was then detected by quantitative real-time PCR.TheΔCT (threshold cycle) value was obtained by subtracting the CT value of 18S rRNA from that of the targeted gene.Results TheΔCT value of CDR2 was significantly lower in fluconazole-resistant isolates than in fluconazolesusceptible isolates (7.52±2.53 vs.9.28±3.15,t=2.367,P<0.05),indicating the expression of CDR2 gene was higher in the fluconazole-resistant isolates than in the fluconazole-susceptible isolates.Conclusion It is likely that the over-expression of CDR2 gene is relevant to fluconazole resistance in Candida albicans.

Key words: MDR, Drug resistance, fungal, Candida albicans, Fluconazole, Genes