Abstract: Acid tolerance and alkaline tolerance of 21 standard strains and 91 clinical strains of Candica spp.were studied and 165 clinical specimens were isolated and identified simultaneously by acid liquid method(PH 1.55)acid-alkaline liquid method(PH 1.55 and pH 8.5) and simplified identification method.The results showed that the acid tolerance(pH 1.55 or pH 1.40)and alkaline tolerance(pH 8.5) of C.alhicans were.more significant than those of other Candida spp.Acid liquid method or aeid-alkaline liquid method dcveloped in the paper can isolate and identify Candida albicans.The former is better,fast,simples accurate and easy to read.

Key words: Candida, albicans