Abstract: A glucose-peptone-yeast extract-glycerol monostearate agar medium containing rapeseed oil was used for culturing the fungi from the lesion scale of 133 patients with tines versicolor. 90.23% of the culture media grew Pityrosporum orbiculare or/and P. ovate successfully. We compared the growth rate, the average day for fungal colonies appearance, morphology of the fungal spores and the constituent ratios of spherical and/or ovoid spores and hyphae in this rapeseed oil medium with other two media which contain sesame oil or olive oil respectively. The results demonstrated that olive oil could be replaced by rapeseed oil (or sesame oil). Hyphae were seen in 35 of 214 samples (16.36%) under the microscope. The relationship between the epithelial cells of skin and hyphae and the influence of atmospheric temperature in which the scales were taken on the fungal micromorphology were discussed.