Abstract: Objective To report a mycologic study of Geotnchum silvicola isolated from a case of foot ulcer.Methods In order to identify the fungal isolate from a 78-year-old male patient with foot ulcer,direct microscopic examination,fungal culture,scanning electron microscopic examination and DNA sequencing were carried out;API 20C AUX yeast identification system was applied.Moreover,mouse inoculation and drug sensitivity test were performed.Results On Sabouraud's agar at 27℃,the culture of the isolate showed yeast-like milky colonies,which were fiat,dry,and non-adherent.Microscopically,many thick,hyaline and multiseptate hyphae,as well as square or rectangular spores were seen.The fungus was consistent with G.penicillatam as assessed by the API 20C AUX system.By the analysis of the sequence of DI/D2 26S rRNA gene,the isolate showed a homology of 99.1% to G.silvicola model strain UFMG-354-2,and 99.8% to MTCC39474.This strain was highly sensitive to fluconazol,itraconazole,clotrimazole,amphotericin B and nystatin.Its pathogenicity was confirmed by mouse inoculation test.The skin lesions were remarkably improved after two weeks of treatment with itraconazole.Conclusion The isolate from this case of foot ulcer is identified as G.silvicola is reported,which is sensitive to itraconazole.

Key words: Mycoses, Geotrichum silvicola, Skin ulcer