中华皮肤科杂志

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白癜风患者调节性T细胞及单核细胞趋化蛋白-1及可溶性细胞间黏附分子-1的检测

宋秀祖, 许爱娥, 周荷芬   

  1. 杭州市第三人民医院皮肤科 310009
  • 收稿日期:2005-07-20 出版日期:2006-02-15 发布日期:2006-02-15
  • 基金资助:
    国家中医药管理局资助课题(04-05JP29)

The expression of MCP-1 and sICAM-1 in skin tissue fluid and regulatory T cells in blood of vitiligo patients in stable stage

SONG Xiu-zu, XU Ai-e, ZHOU He-fen   

  1. The Third Hospital of Hangzhou, Hangzhou 310009, China
  • Received:2005-07-20 Online:2006-02-15 Published:2006-02-15

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摘要： 目的 探讨稳定期白癜风患者白斑与非白斑处皮肤单核细胞趋化蛋白-1(MCP-1)及可溶性细胞间黏附分子-1(sICAM-1)的变化以及血液中调节性T细胞的表达变化.方法 稳定期白癜风患者进行负压吸疱移植治疗,收集白斑及正常皮肤的疱液,用ELISA法检测皮肤组织液中MCP-1及sICAM-1的水平;收集稳定期白癜风患者血液与正常人比较,流式细胞仪观察调节性T细胞的变化.结果 稳定期白癜风患者,血液中调节性T细胞的表达与正常人差异无统计学意义;寻常型白癜风患者局部白斑与非白斑处皮肤吸引疱疱液MCP-1及sICAM-1水平比较均显著增高,经统计学分析,差异有统计学意义.节段型白癜风白斑与非白斑处MCP-1及sICAM-1的水平差异无统计学意义.结论 寻常型稳定期白癜风患者局部白斑皮肤微环境仍处于免疫异常状态,移植治疗的失败可能与局部微环境异常有关.

关键词: 白癜风, T淋巴细胞, 单核细胞化学吸引蛋白质1, 胞间粘附分子1

Abstract: Objectives To study the difference in the expression of monocyte chemoattractant protein-1 (MCP-1) and soluble intercellular adhesion molecule-1 (sICAM-1) between vitiliginous and non-vitiliginous patches in patients with stable vitiligo,and to compare the number of peripheral regulatory T cells between vitiligo patients in stable stage and normal controls.Methods Suction blister fluid was collected at 1 to 3 h after the suction.The expression of MCP-1 and sICAM-1 in skin tissue fluid was detected with ELISA.The number of regulatory T cells in the blood of vitiligo patients and controls was detected by flow cytometry.Results There was no significant difference in the number of regulatory T cells between vitiligo patients and normal controls.The expression of MCP-1 and sICAM-1 in skin tissue fluid was significantly higher in vitiliginous skin than that in non-vitiliginous patches in patients with common type vitiligo,while there was no significant difference between the two kinds of patches in patients with segmental type vitiligo.Conclusions The immune function is abnormal in vitiliginous skin of the common type vitiligo patients in stable stage,which might explain the lack of success in transplantation for this disease.

Key words: Vitiligo, T-lymphocytes, Monocyte chemoattractant protein-1, Intercellular adhesion molecule-1