中华皮肤科杂志

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降钙素基因相关肽对HaCaT细胞表达血管内皮生长因子的调控

于晓静, 李春阳   

  1. 山东大学齐鲁医院皮肤科 济南 250012
  • 收稿日期:2006-02-13 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    山东省自然科学基金资助项目(Y2005C67)

Calcitonin gene-related peptide regulates the expression of vascular endothelial growth factor in hu-man HaCaT keratinocytes

YU Xiao-jing, LI Chun-yang   

  1. Department of Dermatology, Shandong University Qilu Hospital, Jinan 250012, China
  • Received:2006-02-13 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨降钙素基因相关肽(CGRP)对角质形成细胞表达和分泌血管内皮生长因子(VEGF)的调控.方法 用实时定量PCR(RT-PCR)方法检测CGRP、CGRP1型受体拮抗剂CGRP8-37、细胞外信号调节激酶ERK1/2特异性的抑制剂PD98059、p38MAPK特异性拮抗剂SB203580对HaCaT角质形成细胞表达VEGFmRNA水平的影响;用酶联免疫吸附方法检测HaCaT细胞分泌至培养液中VEGF蛋白的水平.结果 CGRP时间依赖性地促进HaCaT细胞表达VEGFmRNA和分泌VEGF蛋白;CGRP8-37和PD98059均可明显抑制CGRP刺激的HaCaT细胞表达和分泌VEGF,SB203580不能减弱CGRP刺激的VEGF表达和分泌.结论 CGRP可以上调HaCaT细胞表达和分泌VEGF,CGRP1型受体及其相关的ERK1/2信号通路参与其调控.

关键词: 降钙素基因相关肽, 血管内皮生长因子类, HaCaT细胞

Abstract: Objective To investigate the relationship between calcitonin gene-related peptide (CGRP) and angiogenesis-related vascular endothelial growth factor (VEGF) expressed in HaCaT keratinocytes.Methods The levels of VEGF mRNA and protein were determined by real-time PCR and ELISA,respectively,in human HaCaT keratinocytes with or without pretreatment with CGRP receptor antagonist (CGRP8-37) or extracellular signal-regulated kinases 1/2 (ERK1/2) inhibitor (PD98059) or p38 mitogen-activated protein kinase p38MAPK inhibitor (SB203580).Results The levels of VEGF mRNA and protein were significantly increased in a time-dependent manner by the pretreatment with CGRP.However,the phenomenon was abrogated by the pretreatment with CGRP8-37 or PD98059.The SB203580 had no effect on the expression of VEGF mRNA and protein.Conclusion CGRP upregulates the expression of VEGF in human HaCaT keratinocytes via CGRP receptor and activation of ERK1/2 pathway.

Key words: Calcitonin gene-related peptide, Vascular endothelial growth factors, HaCaT cells