中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (1): 47-49.

• 论著 • 上一篇    下一篇

Kaposi肉瘤的组织病理学特征观察

张树华1, 阮幼冰2, 武忠弼2, J. N. Kitinya3, 马云1, 朱玉红1, 吕增华1   

  1. 1. 山东滨州医学院病理学教研室, 256603;
    2. 华中科技大学同济医学院病理系;
    3. Pathological Department, Muhimbili Medical Center, Dares Salaam, Tanzania
  • 收稿日期:2004-01-15 出版日期:2005-01-15 发布日期:2005-01-15

Study on Histopathological Characteristics of Kaposi's Sarcoma

ZHANG Shu-hua1, RUAN You-bing2, WU Zhong-bi2, J. NKitinya3, MA Yun1, ZHU Yu-hong1, LÜ Zeng-hua1   

  1. Department of Pathology, Binzhou Medical College, Binzhou, Shandong 256603, China
  • Received:2004-01-15 Online:2005-01-15 Published:2005-01-15

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摘要： 目的 探讨不同组织学类型Kaposi肉瘤(KS)的表型特征。方法 用形态学、免疫组化、原位杂交和DNA倍体分析方法对不同组织学类型30例共38份样本KS的表型特征作了观察。结果 根据KS病变组织内血管成分和梭形细胞的比例分为血管瘤样型(早期)11份、梭形细胞型(晚期)14份和混合型(中期)13份。CD34和FⅧRAg在血管瘤样型表达强,梭形细胞型表达弱(P<0.01)。p53和c-met蛋白在血管瘤样型表达弱,梭形细胞型表达强(P<0.01);c-metmRNA表达与c-met蛋白相同,p53mRNA表达在血管瘤样型高于梭形细胞型。c-erbB-2在梭形细胞的表达高于血管瘤样型(P<0.01)。梭形细胞型PCNA指数高于血管瘤样型(P<0.01)。DNA倍体分析结果显示KS细胞皆为双倍体。结论 血管瘤样型可能为良性病变,梭形细胞型为恶性病变,混合型为良性向恶性的过渡性病变或称交界性病变。

关键词: 表型, 抗原,CD34, 蛋白质p53, 原癌基因蛋白质c-met, 受体,c-erbB-2, 肉瘤,卡波西

Abstract: Objective To study the phenotypic characteristics of Kaposi's sarcoma(KS) in different histological types. Methods Phenotypic characteristics of KS in different histological types were studied with morphology, immunohistochemistry, hybridization in situ, and DNA ploidy analysis. Results KS was classified into three types, i.e., angiomatous type (early stage, 11 specimens), spindle cell type (late stage, 14 specimens), and mixed type (mid stage, 13 specimens) according to the proportion of blood vessels and spindle cells in the tumor tissue. CD34 and FⅧRAg expression was significantly different among three types(P<0.01), strong in the angiomatous type, weak in the spindle cell type. The expression of p53 and c-met protein was weak in the angiomatous type, and strong in the spindle cell type(P<0.01). The expression of c-met mRNA in three types was similar to the c-met protein, but the expression of p53 mRNA was stronger in angiomatous type than that in spindle all type. The expression of c-erbB-2 in spindle cell type was stronger than that in angiomatous type (P<0.01). The index of PCNA immunostanining was significantly higher in the spindle cell type than that in the angiomatous type(P<0.01). DNA ploidy analysis showed that almost all KS cells were diploidy. Conclusions Phenotypic characteristics of KS are varied in their different development stages, which implies that angiomatous stage may be a benign lesion, spindle cell stage a malignant lesion, and mixed stage a transition lesion (or borderline lesion).

Key words: Sarcoma, Kaposi, Phenotype, Antigen, CD34, Protein p53, Proto-oncogene protein c-met, Receptor, c-erbB-2