中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (2): 74-76.

• 论著 • 上一篇    下一篇

成人正常皮肤CD68阳性单核-巨噬细胞分布的研究

郭英军, 赵玉铭, 王雅坤, 肖毅, 魏群, 陈洪铎   

  1. 中国医科大学附属第一医院 沈阳 110001
  • 收稿日期:2004-02-14 出版日期:2005-02-15 发布日期:2005-02-15
  • 通讯作者: 赵玉铭,E-mail:ymzhao_sy@yahoo.com E-mail:ymzhao_sy@yahoo.com

Distribution of CD68+ Monocytes/Macrophages in Human Normal Dermis

GUO Ying-jun, ZHAO Yu-ming, WANG Ya-kun, XIAO Yi, WEI Qun, CHEN Hong-duo   

  1. Department of Dermatology, No 1 Hospital of China Medical University, Shenyang 110001, China
  • Received:2004-02-14 Online:2005-02-15 Published:2005-02-15

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摘要： 目的 观察正常真皮内CD68阳性单核-巨噬细胞的分布、形态和密度。方法 正常成人8例,每例均取面部、躯干、四肢近端、四肢远端、手掌和足跖6个部位皮肤进行表皮铺片和纵行与水平连续切片。CD68单克隆抗体染色,观察单核-巨噬细胞的分布。结果 真皮浅层CD68阳性细胞可表现为树枝状及非树枝状,呈网状分布。真皮浅层CD68阳性细胞密度为:四肢远端(562±230)个/mm2,腹部(517±162)个/mm2,面部(509±235)个/mm2,手掌(507±192)个/mm2,四肢近端(472±138)个/mm2,足跖(361±78)个/mm2。真皮深层CD68阳性细胞密度低,多为树枝状,散在于胶原纤维间。结论 CD68阳性单核-巨噬细胞在真皮浅层形成较致密网状分布。提示单核-巨噬细胞在真皮内有明确的方向性,其防御的方向是穿透表皮进入真皮的入侵物。

关键词: 真皮, 单核细胞, 巨噬细胞

Abstract: Objective To study the distribution, morphology and density of CD68+ monocytes/macro,phages in normal human dermis. Methods Normal skin specimens from 6 sites including face, trunk, proximal limbs, distal limbs, palms and soles of 8 adults were collected. The epidermal sheets, horizontal and longitudinal sections of the specimens were stained with an ABC immunoperoxidase procedure with anti-CD68 monoclonal antibodies. Results The CD68+ cells were detected within the superficial dermis with various densities:562±230/mm2 at distal limbs, 517±162/mm2 at trunk, 509±235/mm2 at face, 507±192/mm2 at palms, 472±138/mm2 at proximal limbs and 361±78/mm2 at soles. Lower densities of CD68+ cells with dendritic morphology were found in the deep (reticular) dermis, and dispersed among collagen bundles. Conclusions There are CD68+ monocytes and macrophages in the superficial dermis, forming a relatively dense network of defense. Such a distribution might indicate the clear polarity of the CD68+ monocytes/macrophages in the dermis, with their direction of defense towards the dermal-epidermal junction.

Key words: Dermis, Monocytes, Macropages