中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 43-45.

• 研究报道 • 上一篇    下一篇

系统性红斑狼疮患者血管内皮祖细胞测定

陈宏1,赵会娟2,门剑龙3   

  1. 1. 天津市人民医院皮肤科
    2. 天津大学精仪学院
    3. 天津医科大学总医院
  • 收稿日期:2012-01-18 修回日期:2012-08-15 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 陈宏 E-mail:baixue20042006@126.com

Detection of vascular endothelial progenitor cells in patients with systemic lupus erythematosus

  • Received:2012-01-18 Revised:2012-08-15 Online:2013-01-15 Published:2013-01-01
  • Contact: Hong CHEN E-mail:baixue20042006@126.com

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摘要： 目的 探讨系统性红斑狼疮（SLE）患者外周血中血管内皮祖细胞的变化。 方法 IL ACL-9000型血液凝固仪测定82例SLE患者和50例健康对照vW因子抗原含量（vWF:Ag）。流式细胞仪分析外周血中内皮祖细胞（endothelial progenitor cells，EPC）和循环内皮细胞（circulating endothelial cell，CEC）的水平。用线性相关分析检测vWF:Ag、CEC、EPC等指标之间的相关性。 结果 SLE活动期患者外周血每20万个单一核细胞中CD34+细胞、CD133+细胞、CD34+CD133+双阳性细胞数（35.4 ± 16.7、86.5 ± 32.1、361.3 ± 176.4）分别高于稳定期患者（17.1 ± 10.9、28.7 ± 21.5、107.2 ± 44.3）和健康人（13.8 ± 9.6、11.2 ± 5.5、92.3 ± 50.5），差异均有统计学意义（P值均 < 0.01）。与稳定期患者比较，SLE活动期患者外周血每20万个单一核细胞中EPC数（361.3 ± 176.4）和vWF:Ag水平（438.9 ± 205.3）%增高，差异有统计学意义（P值均 < 0.01）；CEC差异无统计学意义（P > 0.05）。稳定期患者各项指标与对照组间无统计学意义（P值均 > 0.05）。SLE患者活动期EPC与vWF:Ag呈正相关（r = 0.67，P < 0.01），与CEC无统计学相关性（P > 0.05）。 结论 SLE患者外周血中EPC数量与血管损伤标志物（vWF:Ag）水平密切相关，表明EPC数量改变可以作为评价病情活动的标志物之一。

关键词: 红斑狼疮，系统性, 内皮细胞, 干细胞

Abstract: CHEN Hong*, ZHAO Hui-juan, MEN Jian-long. *Department of Biomedical Engineering, College of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300120, China 【Abstract】 Objective To characterize the alteration in peripheral blood endothelial progenitor cells （EPCs） of patients with systemic lupus erythematosus （SLE）. Methods Venous blood samples were obtained from 82 female patients with SLE aged （35 ± 10） years and 50 healthy female controls aged （35 ± 13） years. ACL 9000 automated coagulation analyzer was used to determine the level of Von Willebrand factor antigen （vWF Ag）. Flow cytometry was performed to detect peripheral blood EPCs and circulating endothelial cells （CECs）. Analysis of variance was performed to assess the differences in these parameters between patients with active and stable SLE and the controls, and Pearson correlation analysis was conducted to evaluate the relationship between these parameters. Results The number of CD34+ cells, CD133+ cells and CD34+CD133+ cells per 200 000 peripheral blood mononuclear cells was 35.4 ± 16.7, 86.5 ± 32.1 and 361.3 ± 176.4 in patients with active SLE, significantly higher than that in the patients with stable SLE （17.1 ± 10.9, 28.7 ± 21.5, 107.2 ± 44.3, respectively, all P < 0.01）） and the controls （13.8 ± 9.6, 11.2 ± 5.5, 92.3 ± 50.5, respectively, all P < 0.01）. The patients with active SLE exhibited an elevated level of vWF Ag （438.9% ± 205.3% vs. 130.2% ± 51.5%, P < 0.01）, an increased number of EPCs （361.3 ± 176.4 vs. 107.2 ± 44.3, P < 0.01） but a similar number of CECs （127±51 vs. 118 ± 39, P > 0.05） per 200 000 peripheral blood mononuclear cells compared with the healthy controls. No significant differences were observed in these parameters between the patients with stable SLE and the controls （all P > 0.05）. The number of EPCs was positively correlated with the level of vWF Ag （r = 0.67, P < 0.01）, but uncorrelated with the number of CECs （P > 0.05） in patients with active SLE. Conclusions The quantity of EPCs in peripheral blood is closely correlated with the level of the vascular injury marker vWF Ag, hinting that the number of EPCs can serve as a useful marker of disease severity.

Key words: endothelial cell