中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (4): 211-212,268.

• 论著 • 上一篇    下一篇

麻风皮损石蜡切片中酚糖脂抗原表达

曾学思1, 王铁生2, 李阿梅1, 桑红桂1, 刘季和3   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京, 210042;
    2. 江苏省皮肤病防治研究所;
    3. 指导者
  • 收稿日期:1993-07-10 修回日期:1993-10-08 出版日期:1994-08-15 发布日期:1994-08-15
  • 基金资助:
    国家自然科学基金资助课题

Demonstration of PGL-I Antigens in Paraffin Section of Leprosy Skin Lesions

ZENG Xue-Si1, WANG Tie Sheng2, LI A-Mei1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1993-07-10 Revised:1993-10-08 Online:1994-08-15 Published:1994-08-15

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摘要： 用免疫组化染色技术检测了67例各型麻风患者皮肤活检石蜡切片中酚糖脂(PGL-I)抗原的表达,除2例结核样型外有65例标本PGL-I抗原呈阳性表达.麻风杆菌和有关的可溶性抗原染色在形态学上呈5种染色模式.在瘤型、偏瘤型和中间界线类麻风中,PGL-I阳性与抗酸染色阳性基本一致.而在结核样型和偏结核样型麻风中,PGL-I阳性高于抗酸染色.本研究结果表明免疫组化技术有较高的特异性和敏感性,为麻风病理学诊断和病因学研究提供了新方法.

关键词: 麻风, 抗原, 酚糖脂

Abstract: An investigation on the demonstration of PGL-I antigens in sixty-seven paraffin embedded skin biopsies taken from leprosy patients who covered the whole spectrum of the disease.The PGL-I antigen was demonstrated in sixty-five leprosy biopsy specimens except two from TT patients,by immunohistochemical staining technique.The antigens were identified as in tracytoplasmic bacillary staining,and M.leprae and re lated soluble antigen staining,were displayed morphologically in five staining patterns.In LL, BL and BB cases, the number of specimens with positive PGL-1 was basically parallel to the positivity of acidfast staining, while in the lesions of TT and BT, the positive rate of PGL-I was higher than that of acidfast staining.The results indicate that the specificity and sensitivity of the immunohistochemical staining technique used in this study are suitable for both the pathologic diagnosis and the study of the pathogenesis of leprosy.

Key words: Leprosy, Antibodies, An,gens,PGL-I