中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (3): 164-166.

• 论著 • 上一篇    下一篇

从人角阮细胞cDNA文库中扩增160000类天疱疮抗原片段

李冠群, 朱学骏   

  1. 北京医科大学第一医院皮肤科, 100034
  • 收稿日期:1994-07-04 修回日期:1994-11-07 出版日期:1995-06-15 发布日期:1995-06-15
  • 基金资助:
    卫生部基金资助课题

Amplification of 160 000 Bullous Pemphigoid Antigen cDNA Fragment from Human Keratinocyte Library

Li Guanqun, Zhu Xuejun   

  1. Department of Dermatology, First Teaching Hospital, Beijing Medical University, Beijing 100034
  • Received:1994-07-04 Revised:1994-11-07 Online:1995-06-15 Published:1995-06-15

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摘要： 大疱性类天疱疮(BP)有两个自身抗原。本研究根据已公布的cDNA序列,设计了一对引物,以人角朊细胞cDNA文库DNA为模板,成功地扩增出160000BP抗原氨基端一段长588bq的片段,进一步从分子水平证实BP抗原由角阮细胞合成;而直接以表皮DNA为模板则未能扩出这一片段,提示BP抗原的有效基因片段可能被一些内含子隔开。扩增出的这一片段将用于制备cDNA探针及重组抗原的研究,从分子水平来探索BP的自身免疫机制。

关键词: 大疱性类天疱疮, 聚合酶链反应

Abstract: Bullous pemphigoid antibodies can recognize two autoantigens-BPAG1 (220 000-240 000) and BPAG2 (160 000-180 000). With two specific primers, we successfully amplified a 588bp cDNA fragment of 160 000 BP antigen from human keratinocyte cDNA library in this paper. The result confirmed that lip antigens were synthesized by keratinocyte at a molecular level. This cDNA fragment could not be amplified with epidermal DNA Templets. We suggest that BP antigen gene may be separated by some introns. We will prepare cDNA probes and recombinant antigens using PCR product in order to elicit autoimmunity mechanism of BP.

Key words: Bullous pemphigoid, Polymerase chain reaction