中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (5): 296-298.

• 论著 • 上一篇    下一篇

Percoll密度梯度离心技术分离纯化豚鼠表皮Langerhans细胞

郑茂荣, 范青源, 谢勇, 牟贤龙, 方跃明, 顾美芳   

  1. 第二军医大学长海医院皮肤科, 上海 200433
  • 收稿日期:1994-09-12 修回日期:1995-06-05 出版日期:1995-10-15 发布日期:1995-10-15
  • 基金资助:
    国家自然科学基金资助课题

Isolaton and Purification of Epidermal Langerhans Cells from Guinea-pig with Percoll Density Gradient Centrifugation Technique

Zheng Maorong, Fan Qingyuan, Xie Yong   

  1. The Affiliated Changhai Hospital, Second Military Medical University, Shanghai 200433
  • Received:1994-09-12 Revised:1995-06-05 Online:1995-10-15 Published:1995-10-15

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摘要： 表皮Langerhans细胞(LC)仅占表皮细胞的2%～4%,但在免疫系统中占重要作用.为了对LC的表型、功能特点及分子生物学水平等作进一步研究,有必要获得高纯化率且具有活性的LC.我们采用连续、间断Percoll密度梯度离心相结合的方法分离、纯化豚鼠表皮LC.结果表明LC纯化率超过60%,产率超过80%,细胞活性超过90%.自体混合白细胞应答检测证实纯化的LC仍保持功能活性.该方法简单、快速、价廉,且获得非表面标记性的LC.

关键词: Langerhans细胞, 密度梯度离心

Abstract: Although epidermal Langerhans cells (LC) account for only 2%~4% of epidermal cells,they play an important role in the immune system.In order to investigate the phenotype,functional feature,and molecular biology of LC,it is necessary to obtain the LCs with high purity and activity.A combination of continuous and interval Percoll density gradient centrifugation technique was used to isolate and purify epidermal LC from guinea-pig.The results showed that the LC purity rate was more than 60%,yield rate more than 80%,and cell activity more than 90%.Auto-mixed leucocyte response assay showed that the functional activity of LC remained.It is the author's opinion that this method is not only simple,quick,and cheap,but can obtain LC with no surface marker.

Key words: Langerhans cells, Percoll density gradient centrifugation