中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (4): 229-231.

• 论著 • 上一篇    下一篇

天疱疮抗独特型单克隆抗体的研制和鉴定

张春明, 刁庆春, 叶庆佾   

  1. 第三军医大学西南医院皮肤科 重庆 630038
  • 收稿日期:1995-07-20 修回日期:1996-02-08 出版日期:1996-08-15 发布日期:1996-08-15

Preparation and Characterization of Anti-idiotype Monoclonal Antibody Specific to Pemphigus

Zhang Chunming, Diao Qingchun, Ye Qingyi   

  1. Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 630038
  • Received:1995-07-20 Revised:1996-02-08 Online:1996-08-15 Published:1996-08-15

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摘要： 用纯化的天疱疮抗体IgG作为抗原免疫BALB/c小鼠,取免疫鼠的脾细胞与SP2/0骨髓瘤细胞融合,经过反复筛选、克隆化获得两株(PI1,PI2)分泌抗天疱疮独特型的单克隆抗体的杂交瘤细胞系.对其中之一PI1作了深入鉴定.PI1 的抗体亚类属于IgG2b.核型分析结果染色体数为86条.通过免疫荧光竞争抑制试验和ELISA中和抑制试验对PI1进行鉴定,发现PI1既能干扰抗原与天疱疮抗体的结合,又能被不同来源的另一天疱疮抗体所中和.无论竞争性抑制还是中和抑制,都呈现剂量依赖关系.表明PI1杂交瘤细胞所分泌的单克隆抗独特型抗体为结合部位相关型抗体,并对PI1的可能应用意义进行了讨论.

关键词: 天疱疮, 抗体.单克隆

Abstract: We purified IgG in the serum of apatient with pemphigus vulgaris.After immunizing BALB/c mice with purified pemphigus autoantibody(Pab),spleen cells of the mice were fused with a myeloma cell line SP2/0,and we selected two hybridoma cell lines(PI1,PI1) which produced anti-idio-type antibody specific to the pemphigus antibody IgG.The anti-idiotype antibody produced by PI1 hybridoma celline was characterized,it was of the IgG2 bsubclass.PI1 could inhibit the binding of Pab to pemphigus antigen.The binding of PI1 to Pab could beinhibited by the IgG antibody in the sera of other patients with pemphigus.Both competitive inhibition and neutralization inhibition were dose-depen-dent.This suggests that idiotype antibody recognised by PI1 is closely asociated with the paratopy of Pab.The potential usefulness of PI1 in the study of the clinical diagnosis,treatment and immune regulation of patients with pemphigus is discussed.

Key words: Pemphigus, Antibodies,monoclonal