尖锐湿疣患者白介素12、白介素15和肿瘤坏死因子α水平的研究

中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (3): 172-174.

尖锐湿疣患者白介素12、白介素15和肿瘤坏死因子α水平的研究

钱起丰, 莫衍石

深圳市皮肤病研究所, 性病防治中心, 518020

收稿日期:1996-08-05 修回日期:1997-01-07 出版日期:1997-06-15 发布日期:1997-06-15

Study on Serum Levels of Interleukin 12, Interleukin 15 and Tumor Necrosis Factor Alpha in Patients with Condyloma Acuminatum

Qian Qifeng, Mo Yanshi

Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020

Received:1996-08-05 Revised:1997-01-07 Online:1997-06-15 Published:1997-06-15

摘要/Abstract

摘要： 为了研究白介素(IL)12、IL-15和肿瘤坏死因子α(TNFα)在尖锐湿疣发病机理中的作用,采用双抗体夹心酶扩大敏感性免疫检测(EASIA)技术检测了尖锐湿疣患者血清IL-12、IL-15及TNFα的水平。结果发现尖锐湿疣患者IL-12水平比正常人显着降低(P<0.05),IL-15水平虽有降低,但无显着性差异;TNFα水平则无明显改变,说明由于IL-12水平低下而导致一系列细胞免疫反应抑制效应,包括在病毒(特异性抗原)早期感染时,不能有效地启动Th1型细胞对病毒抗原的应答反应以及增强T细胞和自然杀伤细胞活性,在尖锐湿疣发病机理中起着重要作用。

关键词: 尖锐湿疣, 白介素12, 白介素15, 肿瘤坏死因子α

Abstract: In order to study the role of interleukin(IL)12, IL15 and tumor necrosis factor alpha(TNF-α) in the pathogenesis of condyloma acuminatum(CA),we used double antibody sandwich ELISA for the quantitative determination of serum IL12,IL15 and TNF-α levels in 31,38 and 27 patients with CA respectively. The results showed the serum IL12 level was significantly(P<0.05) lower in CA patients than that of controls. The level of IL15 was also lower in patients than that in normals, but the difference was not significant (P>0.05).The TNF-α level in patients was almost the same as the controls.These results indicate that the lower level of IL12 results in inhibitory effects on a series of cell-immune responses,being not able to have an effect on T cell and NK cell activation and initiation of Th1-type cell responses at the very early stages of virus infection,which may play an important role in the pathogenesis of CA.

Key words: Condyloma acuminatum, Interleukin 12, Interleukin 15, Tumor necrosis factor alpha

钱起丰, 莫衍石. 尖锐湿疣患者白介素12、白介素15和肿瘤坏死因子α水平的研究[J]. 中华皮肤科杂志, 1997, 30(3): 172-174.

Qian Qifeng, Mo Yanshi. Study on Serum Levels of Interleukin 12, Interleukin 15 and Tumor Necrosis Factor Alpha in Patients with Condyloma Acuminatum[J]. Chinese Journal of Dermatology, 1997, 30(3): 172-174.

参考文献

1 Kobayashi ML, Fitz M, Ryan M, et al. Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effecs on human lymphocytes. J Exp Med, 1989, 170:827.
2 Chan S H, Perussia B, Gupt a JW, et al. In duction of interferon gamma production by nat ural killer cell stimulatory factor: charact erization of the responder cells and the synergy with other inducers. J Exp Med, 1991, 173:869.
3 Gately MK, Wolit zky A G, Quinn PM, et al. Regulation of human cytolytic lymphocyte responses by interleukin-12. Cell Immunol, 1992, 143:127.
4 Gately MK, Desai BB, Wolitzky A G, et al. Regulation of human lymphocyte proliferation by a heterodimeric cytokine, IL-12 (cytotxic lymphocyte maturation factor). J Im munol, 1991, 147:874.
5 Manet ti R, Parronchi P, Giudizi MG, et al. Natural killer cell stimulatory factor(interleukin-12) [IL-12] induces T helper type 1(Th1)-specific immuneresponses and inhibits the development of IL-4-producing Th cells. J Exp Med,1993, 177:1199.
6 Grabst ei n KH, Eisenman J, Shanebeck K, et al. Cloning of a T cell growth factor that interact swith the chain of the interleukin-2 receptor. Science, 1994, 264:965.
7 Blau velt A, Asada H, Klaus-kovtun V, et al. Interleukin-15 mR\NA is expressed by human keratinocytes, Langerhans cells, blood-derived dendritic cells and is downregu-lated by ult raviolet radiation. J Invest Dermatol, 1996,106:1047.
8 Wolf SF, Temple PA, Kobayashi M, et al. Cloning of cDNA for natural killer cell stimulatory facor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J Immunol, 1991,146:3074.
9 彭曙光编译. IL -12 及其在T h1细胞生成中的作用. 国外医学免疫学分册, 1994, 17:260.
10 郑杭民综述. IL -12-NK细胞刺激因子的研究进展. 上海免疫学杂志, 1994, 14:315.
11 钱起丰. 尖锐湿疣患者血清白介素10水平及其与白介素2关系的研究. 中华皮肤科杂志, 1996, 29:172.
12 Giri JG, A hdieh M, Eisen man J, et al. U tilization of the and chains of the IL-2 receptor by the novel cytokine IL-15. EMBO J, 1994, 13:2822.

[1]	童建波杜蕾蕾曾荣王丽玮刘宇甄段志敏陈青李岷. 烟曲霉对人急性单核细胞白血病细胞系产生肿瘤坏死因子α、活化信号分子p38丝裂原活化蛋白激酶的影响[J]. 中华皮肤科杂志, 2018, 51(9): 653-657.
[2]	黄丹鞠梅常宝珠陈旭张小华朱艳周亚男许南陈崑顾恒. 51例女童尖锐湿疣临床分析[J]. 中华皮肤科杂志, 2018, 51(5): 363-365.
[3]	仝云蕾陈铭龚瑜张玲玲于倩王瑶史玉玲. 重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白联合甲氨蝶呤治疗银屑病及其对白细胞介素17A和肿瘤坏死因子α的影响[J]. 中华皮肤科杂志, 2017, 50(9): 636-640.
[4]	任立汆加杨娥燕华玲王永郭砚哈筱梅朱世文王刚. 黑果枸杞多糖对中波紫外线诱导HaCaT细胞光损伤的保护作用[J]. 中华皮肤科杂志, 2017, 50(7): 512-516.
[5]	陈静思孙晨阳海平甘立强谭春花王华罗晓燕. 过敏性紫癜患者中性粒细胞及其IgA Fc受体对血管内皮细胞凋亡的影响及机制[J]. 中华皮肤科杂志, 2017, 50(11): 795-799.
[6]	赵建光禹志成. 液氮冷冻联合卡介菌多糖核酸治疗尖锐湿疣疗效观察[J]. 中华皮肤科杂志, 2012, 45(9): 677-677.
[7]	郑双进黎兆军樊翌明. 尖锐湿疣组织内血管内皮生长因子和转化生长因子-α表达[J]. 中华皮肤科杂志, 2011, 44(5): 350-351.
[8]	栾红姜丽亚孟宪敏. 女性尖锐湿疣患者宫颈亚临床感染与复发关系的研究[J]. 中华皮肤科杂志, 2011, 44(5): 356-357.
[9]	茅晓红张巍巍陈贤祯程浩. Toll样受体在尖锐湿疣皮损中的表达[J]. 中华皮肤科杂志, 2010, 43(9): 637-640.
[10]	吴意平常建民杨敏鲍迎秋傅裕. 氨基酮戊酸光动力疗法治疗尖锐湿疣的临床观察[J]. 中华皮肤科杂志, 2010, 43(8): 589-590.
[11]	邓列华李璟蓉胡云峰许向前吴焱郭秀枝刘洁范洪涛林泽赵永铿. 尖锐湿疣组织中生存素、环氧合酶-2和血管内皮生长因子的表达及其与血管生成的关系[J]. 中华皮肤科杂志, 2010, 43(11): 766-769.
[12]	王秀丽缪飞张玲琳过明霞张海卿王宏伟. 5-氨基酮戊酸光动力疗法治疗宫颈尖锐湿疣临床研究[J]. 中华皮肤科杂志, 2010, 43(10): 694-697.
[13]	徐娟王京陆学东贺勤. 临床表现为假性湿疣的高危型HPV感染[J]. 中华皮肤科杂志, 2009, 42(5): 303-304.
[14]	周林骆丹. HIF-1α、VEGF及Ang-2在妊娠伴尖锐湿疣组织中的表达[J]. 中华皮肤科杂志, 2009, 42(5): 324-326.
[15]	李其林赵艳阳尚智伟付金玲付金玲李晓辉. 尖锐湿疣初发患者外周血单核细胞Toll样受体3、4、9的表达[J]. 中华皮肤科杂志, 2009, 42(5): 350-350.