中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (3): 170-171.

• 论著 • 上一篇    下一篇

聚合酶链反应检测生殖支原体的研究

张伟云, 王荷英, 叶顺章, 施美琴, 王红春   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:1996-12-02 修回日期:1997-01-29 出版日期:1997-06-15 发布日期:1997-06-15

Detection of Mycoplasma Genitalium by Polymerase Chain Reaction

Zhang Weiyun, Wang Heying, Ye Shunzhang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1996-12-02 Revised:1997-01-29 Online:1997-06-15 Published:1997-06-15

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摘要： 具有致病性的生殖支原体(Mg)生长缓慢,不易培养,而血清学诊断与肺炎支原体又有交叉反应,这给Mg的临床诊断带来困难。为了进一步解决Mg的临床检测问题,本研究采用Mg粘附因子基因序列合成一对引物MgPa-1和MgPa-3,用聚合酶链反应(PCR)方法检测Mg.结果表明,对Mg能产生特异性扩增,片段大小为281bp,而对肺炎支原体、解脲支原体、人型支原体、沙眼衣原体及淋球菌等则不能扩增出任何片段。作者认为,PCR为一种高度特异和敏感性的方法,它为Mg的临床检测提供了重要的手段。我们还对引物浓度、退火温度及模板量对PCR扩增结果的影响进行了初步探讨。

关键词: 生殖支原体, 聚合酶链反应

Abstract: Pathogenic Mycoplasma genitalium multiplies slowly and is difficult to be cultured. Furthermore, M.genitalium(Mg) and M.pneumoniae share common antigens, which can give rise to extensive cross-reactions in serological tests, this makes the diagnosis of Mg infection difficult. In order to solve this problem, we detected Mg by polymerase chain reaction(PCR) method. On the basis of the published nucleotide sequence of M. genitalium adhesion protein gene,two primers,MgPa-1 and MgPa-3,were synthesized. The results showed that amplification of Mg DNA yielded the predicted 281bp fragment, and amplification of M.pneumoniae, U.urealyticum,M.hominis,C.trachomatis and N.gonorrhoeae etc did not yield any fragment. It is the authors′, opinion that PCR is specific and sensitive, and it provides an important method for the laboratory diagnosis of Mg infection.The effects of primer concentration, annealing temperature and quantity of template on the result of amplification were discussed.

Key words: M.genitalium, Polymerase chain reaction