培养人角朊细胞中白介素15的基因表达

摘要/Abstract

摘要： 目的为了探讨白介素15(IL-15)在表皮角朊细胞中的表达及调节.方法应用RT-PCR及Northern杂交方法分析了IL-15在培养的人角朊细胞及鳞状细胞癌细胞系(HSC-5)中的表达,并观察了地塞米松对角朊细胞表达IL-15的影响.结果正常角朊细胞及鳞状细胞癌细胞系细胞均有IL-15 mRNA的表达,10^-6mol/L地塞米松处理培养的角朊细胞后,IL-15 mRNA表达水平明显降低.结论表皮细胞来源的IL-15可能介入某些炎症性皮肤病的发病过程.

关键词: 角蛋白细胞, 白细胞介素15, 印迹法,RNA

Abstract: Objective To study the expression and regulation of interleukin-15 (IL-15) in epidermal keratinocytes. Methods The expression of IL-15 mRNA in cultured normal human keratinocytes (NHKs) and human squamous cell carcinoma cell line (HSC-5) was analysed, and the effect of dexamethasone on IL-15 mRNA expression was studied by using RT-PCR and Northern blotting technique. Results The results showed that both NHKs and HSC-5 expressed IL-15 constitutively. The level of IL-15 mRNA was significantly decreased after the cells were cultured with 10^-6M dexamethasone. Conclusion It is suggested that keratinocyte-derived IL-15 might be involved in the development of certain inflammatory skin diseases.

Key words: Keratinocytes, Interleukin-15, Blotting,Northern

韩钢文, 岩月啓氏, 马圣清, 金子史男. 培养人角朊细胞中白介素15的基因表达[J]. 中华皮肤科杂志, 1998, 31(4): 236-238.doi:

Han Gangwen, Keiji Iwatsuki, Ma Shengqing. The Expression of IL-15 mRNA in Cultured Human Keratinocytes[J]. Chinese Journal of Dermatology, 1998, 31(4): 236-238.doi:

参考文献

1 Grabstein KH, Eisenman J, Shanebeck K, et al. Cloning of a T cell growth factor that interacts with the β chain of the interleukin-2 receptor. Science, 1994,264:965-968.
2 Bamford RN, Battiata AP, Borton JD, et al. Interleukin (IL)15/IL-T productionby the adult T-cell leukemia cell line HuT-102 is associated with a human T-cell lymphotrophic virus type ⅠR region/IL-15 fusion message that lacks many upstream AUGs that normally attenuate IL-15 mRNA translation. Proc Natl Acad SciUSA, 1996,93:2897-2902.
3 Carson WE, Giri JG, Lindemann MJ, et al. Interleukin (IL)15 isa novel cytokinethat activates human natural killer cells via components of the IL-2 receptor.J Exp Med, 1994,180:1395-1403.
4 Lewko WM, Smith TL, Bowman DJ, et al. Interleukin-15 and the growth oftumor-dervied activated T-cells. Cancer Biother, 1995,10:13-20.
5 Armitage RJ, MacDuff BM, Eisenman J, et al. IL-15 has stimulatory activity forthe induction of B cell proliferation and differentiation. J Immunol, 1995,154:483-490.
6 Mohamadzadeh M, Takashima A, Dougherty I, et al. Ultraviolet Bradiation up-regulates the expression of IL-15 in human skin. J Immunol, 1995,155:4492-4496.
7 韩钢文, 周立军, 马圣清, 等. 聚合酶链反应Dig-dUTP掺入法制备桥粒芯蛋白1、3型探针. 中华皮肤科杂志, 1997,30:248-250.
8 Blauvelt A, Asada H, Klaus-Kovtun V, et al. Interleukin-15 mRNA is expressedby human keratinocytes, Langerhans cells, and blood-derived dendritic cells andis downregulated by ultraviolet B radiation. J Invest Dermatol, 1996,106:1047-1052.
9 Sorel M, Cherel M, Dreno B, et al. Production of interleukin-15 by human keratinocytes. Eur J Dermatol, 1996,6:209-212.
10 McInnes IB, Al-Mughales J, Field M, et al. The role of interleukin-15 in T-cell migration and activation in rheumatoid arthritis. Nat Med, 1996,2:175-182.

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