中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 93-95.

• 论著 • 上一篇    下一篇

治疗黄褐斑的中药复方对酪氨酸酶活性的影响

李洪武, 朱文元   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:1999-04-05 发布日期:2000-04-15
  • 基金资助:
    国家自然科学基金资助课题(39570658)

Effects of Traditional Chinese Medicine on Tyrosinase Activity

LI Hongwu, ZHU Wenyuan   

  1. Department of Dermatology, First Affiliated Hospital, Nanjing Med ical University, Nanjing 210029
  • Received:1999-04-05 Published:2000-04-15

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摘要： 目的 研究中药对酪氨酸酶活性的影响,筛选有治疗作用的中药方剂,为应用中药治疗色素障碍性皮肤病提供实验依据。方法 通过计算机排序选定5个应用频次较高的治疗黄褐斑的复方中药,采用蘑菇酪氨酸酶多巴速率氧化法体外测定酪氨酸酶活性。结果 治疗黄褐斑中药复方中六味地黄丸、补中益气汤组酪氨酸酶活性降低(水提物P<0.05,醇提物P>0.05),逍遥丸、桃红四物汤增高(P<0.05,金匮肾气丸降低P>0.05)。六味地黄丸组方中药水提物熟地黄、牡丹皮组酪氨酸酶活性增高,茯苓、山药、山茱萸降低;醇提物中牡丹皮、泽泻增高,茯苓、山药、山茱萸降低。结论 六味地黄丸、补中益气汤使酪氨酸酶活性降低(水提物);六味地黄丸组方中药熟地黄、牡丹皮使酪氨酸酶活性增高,茯苓、山药、山茱萸使酪氨酸酶活性降低,泽泻组醇提物增高,水提物降低。

关键词: 黑变病, 复方(中药), 一元酚单氧酶

Abstract: Objective To investigate the effect of traditional Chinese medicine(TCM) on tyrosinase activity and provide experimental evidence for treating melasma. Methods Tyrosinase activity was measured by the rate of oxidation of DL-dopa. Results Tyrosinase activity decreased in groups of patients with Decoction of Six Drugs Including Rehmannia(Liuwei Dihuang Wan), Decoction for Reinforcing Middle jiao(Buzhong Yiqi Tang) (water extract P<0.05, ethanol extract P>0.05), increased in groups of patients with Modified Ease Pill(Xiaoyao Wan),Decoction of Four Ingredients plus Peach Kernel and Safflower(Taohong Siwu Tang)(P<0.01) and decreased in groups of patients with Bolus for Tonifying the Kidney-qi(Jingui Shunqi Wan).While determined in single component TCM of Liuwei Dihuang Wan, tyrosinase activity increased in groups of patients with water extract of Radix Rehmanniae Praeparatae, Cortex Moutan, decreased in Poria, Rhizoma Dioscoreae, Fructus Corni;and increased in groups of patients with ethanol extract of Cortex Moutan,Rhizoma Alismatis, and decreased in Poria, Rhizoma Dioscoreae, Fructus Corni. Conclusions Tyrosinase activity can be reduced by the treatment of Liuwei Dihuang Wan ,Buzhong Yiqi Tang, Poria, Rhizoma Dioscoreae, Fructus Corni;and increased by the treatment of Radix Rehmanniae, Cortex Moutan. Tyrosinase activity increases in ethanol extract and decreases in water extract of Rhizoma Alismatis.

Key words: Melanosis, Composite(TCD), Monophenol monooxygenase