中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 87-89.

• 论著 • 上一篇    下一篇

银屑病皮损内酪氨酸蛋白激酶活性的初步研究

吕根法, 李文维, 伍津津   

  1. 河南洛阳解放军第五三四医院皮肤科, 重庆 400038
  • 收稿日期:1999-05-29 发布日期:2000-04-15

Preliminary Study on Tyrosine Protein Kinase in Keratinocytes of Psoriatic Lesions

LÜ Genfa, LI Wenwei, WU Jinjin   

  1. Department of Dermatology, Daping Hospital, Third Military Medical University 400038
  • Received:1999-05-29 Published:2000-04-15

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摘要： 目的 探讨酪氨酸蛋白激酶(TPK)在银屑病表皮异常增生中的作用。方法 通过外源性底物磷酸化的方法,测定银屑病进行期皮损角质形成细胞的TPK活性。结果 ①银屑病进行期皮损角质形成细胞基础TPK活性显著增高,且对于转化生长因子-α的刺激,反应性明显增强;②增高的基础TPK活性与角质形成细胞膜表皮生长因子受体的表达量呈明显正相关;同时银屑病角质形成细胞表皮生长因子受体的自磷酸化水平亦显著增高。结论 TPK活性增高,以及对有丝分裂信号刺激的反应增强,可能在表皮的增生中起到重要作用,TPK活性的改变可能主要是由于表皮生长因子受体的表达异常所致,表皮生长因子受体可能在银屑病表皮的异常增生中发挥着重要作用。

关键词: 银屑病, 蛋白质酪氨酸激酶, 受体, 表皮生长因子-尿抑胃素, 转化生长因子α

Abstract: Objective To study the mechanism of abnormal proliferation of keratinocytes in psoriasis. Methods The activity of tyrosine protein kinase (TPK) in the keratinocytes of lesional skin in progressive stage was assayed by phosphorylation of exogenous substrates. Results The basic activity of TPK and autophosphorylation of transforming growth factor(TGF) receptor were significantly increased in the psoriatic lesions in progressive stage.The TPK activity of epidermal growth factor(EGF)-receptor was also significantly increased in the psoriatic lesions after stimulation of TGF-alpha as compared with normal control.There was a positive correlation between the activity of TPK and expression of EGFR in the psoriatic keratinocytes. Conclusions The increased activity of TPK and the high reactivity to the stimulation of growth factors are important in the hyperproliferation of psoriatic keratinocytes.The change of the activity of TPK may result from increased expression of EGF receptors. EGF receptors probably play an important role in keratinocyte hyperproliferation of psoriasis.

Key words: Psoriasis, Protein-Tyrosine kinase, Receptors, epidermal growth factor-urogastrone, Transforming growth factor alpha