中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 77-79.

• 论著 •    下一篇

银屑病患者皮损中细胞凋亡情况的评价

茅晓红1, 程浩1, 孙国均1, 陈智2, 周凯1   

  1. 1. 浙江大学医学院附属第二医院皮肤科, 杭州 310009;
    2. 浙江大学医学院传染病研究所
  • 收稿日期:1999-02-25 发布日期:2000-04-15
  • 基金资助:
    浙江省教委科研项目

Evaluation of Cell Apoptosis in Lesional and Non-lesional Psoriatic Skin

MAO Xiaohong1, CHENG Hao1, SUN Guojun1   

  1. Department of Dermatology, The 2nd Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310009
  • Received:1999-02-25 Published:2000-04-15

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摘要： 目的 探讨细胞凋亡与银屑病的关系。方法 用免疫组化SP法检测银屑病皮损与非皮损中Bax、Fas、Fas-L的表达,并用TUNEL(末端脱氧核苷酰转移酶介导的d-UTP-生物素缺口末端标记)技术检测细胞凋亡情况。结果 与正常人相比,银屑病表皮角质形成细胞,真皮炎症细胞及血管内皮均存在不同程度的上述蛋白的过度表达,伴细胞凋亡增多,部分在治疗后未完全恢复正常。结论 推测银屑病皮损中角质形成细胞凋亡增多是针对细胞过度增殖的一种内在稳态机制,可能与Bax,Fas及Fas-L等凋亡相关分子的异常表达有关,而Fas系统还可能参与银屑病的局部免疫异常。

关键词: 银屑病, 脱噬作用, Bax, 抗原, CD95, Fas-, L

Abstract: Objective To study the relationship between cell apoptosis and psoriasis. Methods The expression of Bax, Fas and Fas-L was studied with immunohistochemical technique(SP), and apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay in lesional and non-lesional psoriatic skin. Results In comparison with normal skin, there was an over-expression of Bax, Fas and Fas-L in epidermal keratinocytes, inflammatory cells and vascular endothelium in psoriatic skin, and accompanied with up-regulation of apoptosis. The above abnormalities were not corrected completely after effective treatment in some cases. Conclusions The increase of apoptotic keratinocytes is possibly one kind of internal homeostatic mechanism against the hyperplasia of epidermal cells, and might relate to abnormal expression of apoptosis-associated proteins Bax, Fas and Fas-L. The Fas system might also take part in unusual local immunoreaction in psoriasis.

Key words: Psoriasis, Apoptosis, Bax Antigens, CD95 Fas-L