中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (3): 169-170.

• 论著 • 上一篇    下一篇

人型支原体gyrA基因突变与耐喹诺酮类药物的关系

向斌, 吴移谋, 尹卫国, 余敏君   

  1. 湖南省衡阳医学院微生物学教研室 421001
  • 收稿日期:1999-08-30 出版日期:2000-06-15 发布日期:2000-06-15
  • 基金资助:
    湖南省教委资助重点课题

Study on gyrA Gene Mutations in Quinolone Resistant Mycoplasma Hominis

XIANG Bin, WU Yimou, YIN Weiguo   

  1. Department of Microbiology, Hengyang Medical College, Hunan 421001
  • Received:1999-08-30 Online:2000-06-15 Published:2000-06-15

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摘要： 目的 探讨gyrA基因在人型支原体(Mh)耐喹诺酮类药物中的突变作用.方法 从100株取自临床的Mh中,通过肉汤稀释法筛选出3株对9种喹诺酮类药物呈交叉耐药的Mh株,进行gyrA基因的PCR扩增,分析产物的核苷酸序列,与标准敏感株MhPG21的gyrA基因进行比较.结果 3株Mh的gyrA基因PCR扩增均得到一350bp的阳性片段,产物核苷酸序列分析发现3株均有113位C→T、168位T→A、183位C→T、261位T→C的突变,其中113位C→T的突变导致其编码的83位丝氨酸转变为亮氨酸,其它突变未改变其编码的氨基酸.结论 gyrA基因中C→T的突变与Mh耐喹诺酮类药物密切相关.

关键词: 支原体,人型, 基因,gyrA, 突变, 喹诺酮类

Abstract: Objective To study the role of gyrA gene in quinolone resistant mutants of Mycoplasma hominis(Mh).Methods Three cross resistant mutants of Mycoplasma hominis(Mh) to 9 quinolones were selected from 100 clinical strains of Mh by broth dilution method.Their gyrA were amplified by PCR,and the nucleotide sequences were compared to those of susceptible strain MhPG21 by DNA sequencing.Results A 350 bp DNA fragment was obtained by PCR.Three resistant mutants contained nucleotide 113 C→T,168 T→A,183 C→T,261 T→C transitions.Only 113 C→T transition lead to substitution of Ser-83 by Leu in the gyrA protein,the others did not lead to the substitution of amino acid.Conclusion These results suggest that a gyrA mutation in Mh at Ser-83 is associated with quinolone resistance.

Key words: Mycoplasma hominis, Genes,gyrA, Mutation, Quinolones