中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (3): 149-151.

• 论著 •    下一篇

贵州省HIV-1感染毒株膜蛋白基因序列测定及流行病学分析

雷世光1, 孙显光1, 邵一鸣2, 邢辉2, 杨宏亚1, 王丁力1, 乐斌1, 李豫1   

  1. 1. 贵州省皮肤病性病防治研究所 贵阳 550001;
    2. 卫生部艾滋病预防与控制中心
  • 收稿日期:1999-11-10 出版日期:2000-06-15 发布日期:2000-06-15
  • 基金资助:
    贵州省自然科学基金

The env Gene Sequencing of HIV-1 Strains and their Epidemic Status in Guizhou Province

LEI Shiguang1, SUN Xianguang1, SHAO Yiming2   

  1. Guizhou Provincial Institute of Dermatology and Venereology, Guiyang 550001
  • Received:1999-11-10 Online:2000-06-15 Published:2000-06-15

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摘要： 目的 揭示贵州省HIV-1感染毒株的亚型分布及其流行态势.方法 用巢式PCR法扩增HIV-1膜蛋白基因(env),并经序列分析.结果 核酸分离成功的11份全血标本中,B亚型7例,C、E亚型各2例;B亚型基因离散率为3.21%±1.4%,流行时间约3年,E和C亚型基因离散率为1.65%和0.98%,流行时间约1年.结论 贵州省HIV-1感染毒株亚型分布广泛,来源复杂,预示今后将以性传播途径为主,防治难度较大.

关键词: HIV-1, 序列分析, 流行病学

Abstract: Objective To reveal the distribution of subtypes of HIV-1 and their epidemic status in Guizhou Province.Methods The provirus DNA of HIV was extracted from the peripheral blood mononuclear cells in 11 HIV-1 infected individuals,the env gene of HIV was analysed by nested PCR and DNA sequencing.Results Among eleven HIV positive blood samples,7 were subtype B (accounting for 64%),2 were subtype C (accounting for 18%),another 2 were subtype E (accounting for 18%).The genic dispersion rate of subtype B was 3.21±1.4%,with 3 years epidemic in Guizhou.The genic dispersion rates among subtype E or C were 1.65% or 0.98%,respectively,with 1 year epidemic in Guizhou.Conclusions There are various subtypes of HIV strains in Guizhou Province,transmitted from different sources,which indicates that sexual transmission will be dominant in the future,and also more difficult to control.

Key words: HIV-1, Sequence analysis, Epidemiology