中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (1): 22-24.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞Caspase-1和Caspase-3mRNA的表达

弓娟琴, 陈志强, 胡兹嘉, 李文忠   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2001-04-05 出版日期:2002-02-15 发布日期:2002-02-15
  • 通讯作者: 陈志强, 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042

mRNA Expression of Caspase-1 and Caspase-3 in the Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus

Gong Juanqin, Chen Zhiqiang, Hu Zijia, LI Wengzhong   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2001-04-05 Online:2002-02-15 Published:2002-02-15

PDF (PC)

83

赞

摘要： 目的 探讨系统性红斑狼疮(SLE)患者外周血淋巴细胞Caspase-1和Caspase-3的表达水平及其与淋巴细胞凋亡的关系。方法 应用逆转录-聚合酶链反应(RT-PCR)检测34例活动期SLE患者和30例正常人外周血单一核细胞(PBMC)中Caspase-1和Caspase-3mRNA的表达水平。结果 活动期SLE患者PBMC中Caspase-1mRNA的平均表达水平(1.20±0.33)明显高于正常人对照组(0.79±0.25),差异有非常显着性(P<0.01);Caspase-3mRNA的平均表达水平(0.87±0.16)高于正常人对照组(0.66±0.10),差异有显着性(P<0.05).活动期SLE患者PBMC中Caspase-1和Cas-pase-3的阳性表达率与正常人对照组相比差异无显着性(P>0.05).结论 活动期SLE患者外周血淋巴细胞Caspase-1和Caspase-3mRNA表达水平增高,Caspase级联反应可能参与了SLE患者淋巴细胞的凋亡过程。

关键词: 红斑狼疮,系统性, Caspase1, Caspase3, 脱噬作用

Abstract: Objective To investigate the mRNA espression level of Caspase-1 and Caspase-3 in the patients with systemic lupus erythematosus(SLE) and its association with lymphocyte apoptosis. Methods Reverse transcription-polymerase reaction (RT-PCR) technique was applied to semiquantitatively analyze the mRNA expression of Caspase-1 and Caspase-3 in the peripheral blood mononuclear cells (PBMC) from 34 active SLE patients and 30 healthy subjects. Results Compared with normal controls, the mean levels of Caspase-1 or Caspase-3 mRNA expression in active SLE group was significantly increased(P<0.01 and P<0.05, respectively). But there was no statistical difference in the positive rate of Caspase-1 and Caspase-3 expression between the active SLE patients and the normal controls (P>0.05). Conclusion There are increased expressin of Caspase-1 and Caspase-3 in the PBMC of SLE patients, which may be involved in the lymphocyte apoptosis of SLE patients.

Key words: Lupus erythematosus, systemic, Caspase 1, Caspase 3, Apoptosis