中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (4): 192-194.

• 论著 • 上一篇    下一篇

鳞状细胞癌细胞系12F细胞中白介素1αmRNA的表达及对紫外线照射的反应

骆丹, 闵玮   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:2002-06-03 出版日期:2003-04-15 发布日期:2003-04-15

IL-1αmRNA Expression and Protein Secretion After Ultraviolet Irradiation in Human Keratinocyte Originated SCC 12F Cells

LUO Dan, MIN Wei   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2002-06-03 Online:2003-04-15 Published:2003-04-15

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摘要： 目的 了解人角质形成细胞(KC)起源的鳞状细胞癌细胞系(SCC)12F细胞自发表达白介素1α(IL-1α)mRNA的能力以及紫外线照射对IL-1α分泌水平的影响。方法 采用RNA印迹法检测IL-1αmRNA的表达水平,采用ELISA方法检测IL-1α蛋白的分泌水平。结果 SCC12F细胞可在正常KC培养体系中自发表达IL-1αmRNA,其表达水平随细胞培养时间的延长而增强,在120h达到高峰值;经中波紫外线(UVB)照射后,IL-1α蛋白的分泌水平既随照射时间增加,亦随照射剂量增加。结论 SCC12F细胞在正常KC培养体系中可自发表达IL-1αmRNA,其IL-1α蛋白的分泌水平对UVB照射呈时效及量效反应。

关键词: 角蛋白细胞, 肿瘤细胞,培养的, 白细胞介素1, 紫外线

Abstract: Objective To investigate IL-1α mRNA expression and protein secretion after ultraviolet irradiation in human keratinocyte(KC)originated SCC 12F cells.Methods Expression of IL-1α mRNA was detected by Northern blot and the secretion level of IL-1α protein was analyzed by ELISA.Results There was a spontaneous expression of IL-1α mRNA in SCC 12F cells in culture system of normal KC,which was increased along with the culture time,and reached the highest expression level in 120 hours.The secretion level of IL-1α protein was increased in a time-and dose-dependent manner in SCC 12F cells after ultraviolet B(UVB)irradiation.Conclusions SCC 12F cells may express IL-1α mRNA spontaneously under regular KC media.The secretion level of IL-1α protein shows both time-and dose-effect after UVB irradiation.

Key words: Keratinocytes, Tumor cells,cultured, Interleukin-1, Ultraviolet rays