中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (12): 738-740.

• 论著 • 上一篇    下一篇

表皮体外重建模型的研究

陆洪光, 郭哲, 崔绍山, 王雅坤, 陈洪铎   

  1. 中国医科大学附属第一医院皮肤科 沈阳 110001
  • 收稿日期:2005-02-21 发布日期:2005-12-15
  • 通讯作者: 陈洪铎,E-mail:hongduochen@hotmail.com E-mail:hongduochen@hotmail.com
  • 基金资助:
    国家自然科学基金资助项目(30571685)

A Study of Reconstructed Human Epidermis Model in vitro

LU Hong-guang, GUO Zhe, CUI Shao- shan, WANG Ya-kun, CHEN Hong-duo   

  1. Department of Dermatology, First Hospital of China Medical University, Shenyang 110001, China
  • Received:2005-02-21 Published:2005-12-15

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摘要： 目的 建立表皮体外重建模型,并对其进行表皮修复的研究。方法 采用去表皮的真皮组织作为真皮替代物,其上接种2mm的微小皮瓣,在培养液中加入表皮生长因子(实验组),应用荧光表皮成像技术观察表皮的生长,组织病理和免疫组化(Ki67)观察表皮的增殖和分化,与无表皮生长因子的基础培养液培养的表皮(对照组)比较。结果 接种后第3天可观察到表皮在真皮组织表面生长,10d左右形成完整表皮结构。表皮生长因子有明显的促表皮移行作用,至第10天表皮生长因子组表皮生长面积(t=3.02,P<0.01)、表皮生长厚度(t=2.87,P<0.01)、有核细胞的细胞层数(t=2.43,P<0.05)、皮突增殖指数(t=2.29,P<0.01)、细胞增殖密度(t=2.33,P<0.05)均显著高于对照组。结论 用去表皮的真皮组织可以诱导表皮的再生、发育和分化。

关键词: 伤口愈合, 组织培养技术, 真皮替代物

Abstract: Objective To establish a reconstructed human epidermis model in vitro, and to study the process of re-epithelialization. Methods A dermal substrate devoid of epidermis was prepared; a 2 mm skin biopsy explant was transplanted onto the dermal substrates. Visualization of epidermal cell migration was carried out by fluorescence imaging. The proliferation and differentiation of the new epithelial cells were observed using histopathological and immunohistochemical staining (Ki67). EGF was added to the culture medium of the experimental samples but not to that of the controls. Results After 3 days of culture, re-epithelialization was observed on the surface of the dermal substrate. A complete structure resembling regular epidermis was noted in 10 days. As compared to control samples, EGF-treated samples had larger area of re-epithelialization (t=3.02, P<0.01), greater thickness (t=2.87,P<0.01), more layers of cells (t=2.43, P<0.05), higher keratinocyte proliferation indices (t=2.29,P<0.01), and higher cell density (t=2.33, P<0.05). Conclusion A reconstructed dermal substrate could induce epidermal regeneration, development and differentiation in vitro.

Key words: Wound healing, Tissue culture techniques, Dermal substrates