中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (12): 732-734.

• 论著 • 上一篇    下一篇

多部位红色毛癣菌感染分离菌株DNA分型研究

陈辉, 刘维达, 沈永年   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2005-01-25 发布日期:2005-12-15
  • 通讯作者: 刘维达,E-mail:liumyco@hotmail.com E-mail:liumyco@hotmail.com

Molecular Typing of Trichophyton rubrum Isolated from Multiple Skin Lesions

CHEN Hui, LIU Wei-da, SHEN Yong-nian   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2005-01-25 Published:2005-12-15

PDF

93

赞

摘要： 目的 探讨多部位红色毛癣菌感染不同部位分离菌株基因型的差异。方法 采用巢式PCR扩增真菌rDNA非转录间隔区(NTS)中Trs-1片段,检测基因多态性。并比较同一患者不同感染部位分离菌株的基因型差异。结果 受试36株菌株按照PCR指纹图共分为10型,基因型分布与感染部位关系不大。在17例受试患者中,8例不同感染部位分离得到的菌株基因型有差异,且与不同感染部位的病程差异无关。结论 多部位红色毛癣菌感染可能为多菌株引起,提示部分多部位皮肤癣菌感染患者不同部位皮肤癣菌感梁的传染源可能来源于外界,而与机体原已存在的感梁灶关系可能不大。

关键词: 分子生物学分型, 发癣菌属, 癣

Abstract: Objective To determine whether Trichophyton rubrum isolated from different lesions in the same patient is of different strains. Methods DNA was extracted from the isolates, then subjected to a polymerase chain reaction-based typing which analyzed the number of repetitive elements in the non-transcribed spacer region of the ribosomal DNA gene repeats. Results Thirty-six strains of T. rubrum were isolated from 17 patients with fungal infection on multiple sites. All strains could be classified into 10 genotypes. The genotype distribution was unrelated to sites of infection. It happened to 8 of the 17 patients that multiple genotypes were involved in T. rubrum infection on different sites in the same body. Conclusion The study shows that multiple genotypes are involved in T. rubrum infection on different sites in the same patient, suggesting external sources of infection rather than infection from a different site in the same individual.

Key words: Molecular strain typing, Trichophyton, Tinea