中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (9): 506-508.

• 论著 • 上一篇    下一篇

恶性黑素瘤和普通痣细胞痣细胞外信号调节激酶途径相关蛋白的研究

林彤, 孙建方, 曾学思, 桑红桂, 李阿梅, 赵亮   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2003-10-15 出版日期:2004-09-15 发布日期:2004-09-15
  • 通讯作者: 孙建方,E-mail:sunjianf@jlonline.com E-mail:sunjianf@jlonline.com

The Study of ERK Pathway Related Protein Expression in Malignant Melanoma and Ordinary Nevi

LIN Tong, SUN Jian-fang, ZENG Xue-si, SANG Hong-gui, LI A-mei, ZHAO Liang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2003-10-15 Online:2004-09-15 Published:2004-09-15

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摘要： 目的 探讨恶性黑素瘤(MM)和普通痣细胞痣细胞外信号调节激酶(ERK)途径相关蛋白ERK1和ERK2以及细胞周期素D1(Cyclin D1)蛋白的表达情况,分析这3种蛋白与MM的临床病理关系。方法 采用免疫组化SP法对30例MM和13例普通痣细胞痣中ERK1、ERK2和Cyclin D1蛋白的表达进行检测,并进行临床病理相关性分析。结果 ERK1、ERK2和Cyclin D1在MM组织中表达较普通痣细胞痣组织明显升高(P<0.01),ERK1、ERK2和Cyclin D1与MM的Clark分级和Breslow厚度无明显相关性,ERK1和ERK2与Cyclin D1在MM组织中表达显著相关。结论 MM中ERK途径得到了过度激活,并可能促使Cyclin D1表达增加,因此它们在MM的发病机制中可能起到一定作用,而它们在普通痣细胞中仅在A型痣细胞表达增加。

关键词: 黑色素瘤, 痣, 有丝分裂素激活蛋白激酶类, 细胞周期蛋白D1

Abstract: Objective To investigate the roles of extracellular signal-regulated kinase (ERK)1, ERK2 and Cyclin D1 proteins in malignant melanoma (MM) and ordinary nevus. Methods By SP immunohistochemical method, the expression of ERK1, ERK2 and Cyclin D1 proteins was detected in 30 cases of MM and 13 cases of ordinary nevi. Results The expression of ERK1, ERK2 and Cyclin D1 proteins was higher in MMs than that in ordinary nevi (P<0.01). The expression of ERK1, ERK2 and Cyclin D1 was not correlated with Clark level and Breslow thickness. The expression of ERK1 and ERK2 was correlated with that of Cyclin D1. Conclusions ERK pathway is activated in MM and may result in Cyclin D1 over-expression, while in ordinary nevi, only expression of type A nevus cell is increased.

Key words: Melanoma, Nevus, Mitogen-activated protein kinases, Cyclin D1