中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (9): 640-641.

• 短篇论著 • 上一篇    下一篇

结合珠蛋白对特应性皮炎患儿外周血单一核细胞产生IFN-γ和IL-4细胞因子的影响

李萍,李建红深圳,刘建中,冯进云,崔荣,刘启文   

  1. 深圳市儿童医院皮肤科
  • 收稿日期:2008-09-01 修回日期:2008-12-13 出版日期:2009-09-15 发布日期:2012-03-21
  • 通讯作者: 李萍 E-mail:liping20081110@126.com
  • 作者简介:李萍（1971-），女，汉族，籍贯：河北省井陉县，副主任医师，医学博士，研究方向：免疫性及变态反应性皮肤病
    简历：1994.7-2004.4 山西医科大学附属第二医院皮肤性病科工作                 1997.9-2000.7 山西医科大学皮肤性病学专业 硕士                    2000.9-2003.7 中国医科大学皮肤性病学专业 博士             2004.4-至今   深圳市儿童医院皮肤科工作
  • 基金资助:

    市(厅)级基金（编号）

Effect of haptoglobin on the production of interferon-γ and interleukin-4 by peripheral blood mononuclear cells from patients with atopic dermatitis

  • Received:2008-09-01 Revised:2008-12-13 Online:2009-09-15 Published:2012-03-21
  • About author:LI Ping (1971-), female,  Ph.D.  ,  Associate professor. Specialized in immnuological and allergic skin diseasesJul. 1994 - Apr.2004 :  Department of  dermatology, the second hospital  of  Shanxi medical university, Physican Sep. 1997- Jul. 2000 : Shanxi medical university  specialized in dermatology , M.D.Sep. 2000- Jul. 2003:  China medical university specialized in dermatology, Ph.D.Apr.2004- now :   Department of dermatology , Shenzhen children hospital , associate professor

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摘要：

【摘要】 目的 观察不同浓度结合珠蛋白对特应性皮炎患者和正常对照者培养的外周血单个核细胞产生干扰素γ（IFN-γ）和白介素4（IL-4）水平的影响。  方法  按照密度梯度离心法分离、培养30例特应性皮炎患者和15例正常对照者外周血单个核细胞，结合珠蛋白的干预浓度依次为0mg/L 、50 mg/L、150 mg/L和250 mg/L。培养上清中IFN-γ和IL-4水平用ELISA法进行检测，采用RT-PCR结合凝胶图象扫描技术检测培养细胞中IFN-γ和IL-4基因的表达情况。结果   结合珠蛋白浓度为0 mg/L的情况下，患者组与正常对照组对比，培养上清中IFN-γ水平明显降低（P＜0.05），IL-4水平分别 明显增高（P＜0.05）；但相应培养细胞中IFN-γmRNA和IL-4mRNA均明显增高（P＜0.05）。结合珠蛋白浓度为50mg/L、150mg/L和250mg/L的情况下，患者及对照者培养上清中IFN-γ水平依次为524.73±78.40/647.88±19.85 ng/L，527.74±69.92/683.68±75.80 ng/L，533.19±34.24/689.59±75.30 ng/L，逐渐增高；IL-4水平依次为100.57±12.46/94.97±11.82 ng/L，91.32±11.81/90.74±10.93 ng/L，74.56±11.71/74.76±13.58 ng/L，逐渐降低。随着结合珠蛋白浓度的增高，患者组IFN-γ和IL-4mRNA水平均有逐渐降低的趋势，IFN-γmRNA水平与蛋白质水平的变化不一致；正常对照组IFN-γmRNA水平有逐渐增高的趋势，影响规律与患者组相反；而IL-4mRNA水平有逐渐降低的趋势，影响规律与患者组不一致，两者均与蛋白质水平的变化一致。结论 结合珠蛋白在一定浓度下可使特应性皮炎患者外周血单个核细胞产生Th1型细胞因子增加，调节Th1/Th2间的平衡，参与了患者体内的免疫调节。特应性皮炎患者外周血单个核细胞中IFN-γ转录后环节缺陷。

关键词: 白细胞介素4

Abstract:

【Abstract】Objective  To investigate the expression of interferon-gamma (IFN-gamma) and interleukin 4 (IL-4) produced by the peripheral blood mononuclear cells from atopic dermatitis patients and normal controls under different concentrations(0mg/L, 50mg/L, 150mg/L and 250mg/L) of haptoglobin. Methods  The peripheral blood mononuclear cells were separated from 30 patients and 15 healthy controls by gradient centrifugation density, and cultured with or without the presence of haptoglobin for 72 hours. The concentrations of IFN-gamma　and IL-4 in the culture supernatant from all the subjects were measured by enzyme linked immnuosorbent　assay (ELISA). The expression of IFN-gamma and IL-4 gene in cultured peripheral blood mononuclear cells were detected by semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR). Results  With comparison to normal controls, the level of IFN-gamma in patient’s culture supernatant was obviously lower(433.75±87.83 vs 567.76±51.51 ng/L) （P＜0.05）whereas IL-4 was higher (117.88±12.25 vs 101.84±12.86 ng/L ) （P＜0.05） without the presence of haptoglobin. But the expression of IFN-gamma(0.79±0.10 vs 0.56±0.11) and IL-4 (0.74±0.13 vs 0.65±0.10) gene were all increased（P＜0.05）. Haptoglobin resulted in a dose-dependent influence of  IFN-gamma and IL-4 release in culture supernatant from patients and controls. The level of IFN-gamma in the three culture systems（with haptoglobin 50mg/L, 150mg/L and 250mg/L）from patients and controls were 524.73±78.40/647.88±19.85 ng/L, 527.74±69.92/683.68±75.80 ng/L, 533.19±34.24/689.59±75.30 ng/L. The level of IL-4 from that were 100.57±12.46/94.97±11.82 ng/L, 91.32±11.81/90.74±10.93 ng/L, 74.56±11.71/74.76±13.58 ng/L. Which showed the release of IFN-gamma increased but IL-4 decreased. Influence was found on IFN-gamma and IL-4 gene expression in cultured cells from patients and normal controls under different concentrations of  haptoglobin. With haptoglobin increasing, IFN-gamma and IL-4 gene showed the tendency of gradually down in patients. The level of IFN-gamma gene was not consistent with the change of protein. IFN-gamma gene showed the tendency of up in normal controls which contrary to patients. The change on IL-4 gene was consistent with protein level. Conclusion  Haptoglobin plays a modulating role on the Th1/Th2 balance by promoting a dominant Th1 cellular response in atopic dermatitis patients . This points a role of haptoglobin in the immunoregulation of atopic dermatitis. A post-transcriptional defect was found in the peripheral blood mononuclear cells from atopic dermatitis patients in IFN-gamma.