中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (12): 894-895.

• 技术与方法 • 上一篇    下一篇

沙眼衣原体培养时防控耐甲氧西林表皮葡萄球菌污染的探索

江勇1,杨丽娜1,刘原君2,刘全忠2   

  1. 1. 天津医科大学第二医院皮肤性病科
    2. 天津医科大学总医院皮肤性病科
  • 收稿日期:2011-03-18 修回日期:2011-05-07 出版日期:2011-12-15 发布日期:2011-12-02
  • 通讯作者: 刘全忠 E-mail:liuquanzhong@medmail.com.cn
  • 基金资助:

    国家自然科学基金资助项目

Development of a strategy to prevent and control the contamination with meticillin-resistant Staphylococcus epidermidis during the in vitro culture of Chlamydia trachomatis

  • Received:2011-03-18 Revised:2011-05-07 Online:2011-12-15 Published:2011-12-02
  • Contact: quanzhong liu E-mail:liuquanzhong@medmail.com.cn

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摘要：

[目的] 预防和控制沙眼衣原体培养过程中耐甲氧西林表皮葡萄球菌的污染。[材料与方法] 采用纸片扩散法或液体培养法筛选敏感抗菌素，然后用微量稀释法确定最低抑菌浓度和细胞毒作用。[结果] 耐甲氧西林的表皮葡萄球菌球菌产β-内酰胺酶，对头孢噻肟钠、头孢哌酮舒巴坦、头孢曲松钠和庆大霉素、万古霉素敏感。头孢噻肟钠MIC＝50mg/L，包涵体明显减少且体积变小。头孢哌酮舒巴坦、头孢曲松钠MIC分别为20 mg/L、100 mg/L，包涵体消失。庆大霉素、万古霉素MIC分别为3200U/mL、400 mg/L，有明显的细胞毒作用。[结论] 沙眼衣原体培养过程中细菌污染最常见且危害最大，近年常见产耐药酶的表皮葡萄球菌，因此预防污染十分重要。污染标本的处理需要兼顾细胞和细菌、衣原体三方面。第3代头孢类抗菌素能有效杀灭该菌，但是影响包涵体的生长和存活。清除污染菌的理想方案是3200U/mL的庆大霉素作用1至2小时。

关键词: 葡萄球菌

Abstract:

Objective To investigate the method of control and prevention for Meticillin-resistant Staphylococcus epidermidis(MRSE) contamination. Methods Diffusion method and broth method to screening for susceptible antibiotics. Microdilution to determine MIC and cytotoxic effect.Results MRSE was susceptible to cefotaxime sodium, Cefoperazone and Sulbactam and ceftriaxone sodium. MICs were 50,20,100 mg/L respectively.They made the conclusion get less or small.Gentamicin and Vancomycin were susceptible,which MICs were 3200U/mL and 400mg/L ,but they had obvious cytotoxic effect. Conclusion Prevention for MRSE contamination is very important.The third genenation cephalosporin can control the MRSE,but they impact the Chlamydia trachomatis.The favorite treatment is to add the gentamicin for 1-2 hours.