广州地区沙眼衣原体omp1基因分型及突变研究

摘要/Abstract

摘要：

目的探讨不同omp1基因型沙眼衣原体（Ct）在广州地区非淋菌性尿道（宫颈）炎患者中的分布情况。方法从性病和妇科门诊及外展现场采集男性尿道和女性宫颈拭子，提取Ct基因组DNA为模板，巢式PCR扩增Ct omp1基因VD1 ~ VD3并测序分型，分析Ct型别决定簇VD1 ~ VD2区域的氨基酸突变情况。结果共检测1208份拭子，检出Ct 132株，对其中130株进行测序分型，共发现10个omp1基因型，分别为E型38株（29.23%）、D型25株（19.23%）、J型24株（18.46%）、F型21株（16.15%）、G型7株（5.38%）、H型5株（3.85%）、K型5株（3.85%）、B型、Ja型各2株（各1.54%）、I型1株（0.77%）；可见E、D、J、F 4个型别为主要感染型别，共占83%。序列分析发现，D、B、K 3个型别在VD1 ~ VD2区域的氨基酸存在突变。另有2例为混合感染未能确定型别。结论广州地区Ct omp1基因型别感染以E、D、J、 F 4种型别为主，共占83%，Ct B型也发现于男性尿道和女性宫颈。

关键词: 衣原体，沙眼, 细菌外膜蛋白质类, 基因型, 突变

Abstract:

Objective To profile the omp1 genotypes of Chlamydia trachomatis （Ct） in patients with nongonococcal urethritis （cervicitis） in Guangzhou region. Methods Swab samples were obtained from the urethra of males and cervix of females in clinical settings of venereology and gynecology as well as at outreach sites for the prevention and control of sexually transmitted diseases （STDs）. DNA was extracted from the swabs and nested PCR was performed to amplify the variable domain （VD） 1 - 3 of omp1 gene of Ct followed by gene sequencing. The genotypes of Ct were determined based on the amino acid mutation in VD 1 - 2 of omp1 gene. Results Totally, 1208 swabs were collected. Of them, 132 were Ct positive, and 130 positive samples underwent genotyping. Ten omp1 genotypes were determined in total, including serotype E（38, 29.23%）, D （25, 19.23%）, J（24, 18.46%）, F（21, 16.15%）, G（7, 5.38%）, H（5, 3.85%）, K（5, 3.85%）, B（2, 1.54%）, Ja（2, 1.54%）, I（1, 0.77%）. E, D, J and F were the dominant type of Ct in this region, and amounted to 83% of all the Ct isolates. Mutations were observed within VD 1 and 2 of omp1 gene in serotype D, B and K. Serotypes were undetermined for Ct in 2 patients with mixed infection. Conclusions In Guangzhou region, E, D, F and J are the predominant genotypes of Ct, and amount to 83% of all the Ct isolates. Ct serotype B is also observed in the urethra of males and cervix of females in this region.

Key words: Mutation

叶兴东戴向农黎小东余丹丹费实任泽舫朱慧兰. 广州地区沙眼衣原体omp1基因分型及突变研究[J]. 中华皮肤科杂志, 2010, 43(12): 843-846.

参考文献

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