中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (9): 637-640.

• 论著 • 上一篇    下一篇

Toll样受体在尖锐湿疣皮损中的表达

茅晓红1,张巍巍2,陈贤祯3,程浩4   

  1. 1. 浙江大学医学院附属第二医院
    2. 浙江大学医学院附属第二医院皮肤科
    3. 杭州浙江大学医学院附属邵逸夫医院皮肤科
    4. 杭州市浙江大学邵逸夫医院皮肤科
  • 收稿日期:2010-01-13 修回日期:2010-05-09 发布日期:2010-09-10
  • 通讯作者: 程浩 E-mail:hz_chenghao@126.com

Expression of Toll-like receptors in lesions of condyloma acuminatum

  • Received:2010-01-13 Revised:2010-05-09 Published:2010-09-10

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摘要：

目的 探讨尖锐湿疣皮损中Toll样受体（TLR）各亚型的表达。方法 将尖锐湿疣患者的病损组织标本冰冻切片经免疫组化染色检测10种TLR亚型的表达情况，以正常皮肤组织作对照，比较两者TLR表达谱的差异。结果 正常表皮角质层和颗粒层TLR6、9呈强阳性表达，TLR1、3、10呈中等强度表达，TLR2、4、5、7、8弱表达，棘层和基底层细胞不表达或弱表达TLR；尖锐湿疣皮损表皮中除基底层弱表达或不表达TLR外，其余细胞层TLR1、3、6、9强阳性表达，TLR4、8、10中等强度表达，TLR2、5、7弱表达。两组皮肤的真皮层均未见明显TLR阳性细胞。结论 尖锐湿疣皮损增生的角质形成细胞中TLR表达较正常皮肤明显增高，尤以TLR1、3、6、9亚型为著，可能与HPV感染启动机体抗病毒免疫有关。

关键词: Toll样受体, 人乳头瘤病毒, 尖锐湿疣

Abstract:

Objective To measure the expressions of Toll like receptors （TLRs） in lesions of condyloma acuminatum （CA）. Methods Tissue samples were resected from lesions of 25 patients with CA and normal skin of 16 human controls. Frozen sections were prepared and the expressions of TLRs 1 - 10 were measured by immunohistochemical staining. Results In normal skin samples, there was a strong expression of TLR 6 and 9, medium expression of TLR 1, 3, 10 and weak expression of TLR 2, 4, 5, 7, 8 in the stratum corneum and stratum granulosum, but a weak or negative expression of TLRs in the stratum spinosum and stratum basale. In contrast, a strong expression of TLR 1, 3, 6, 9, medium expression of TLR 4, 8, 10 and weak expression of TLR 2, 5, 7 were detected in keratinocytes of almost all epidermal layers except for stratum basale in CA lesions. Conclusions The expressions of TLRs, especially TLR 1, 3, 6, 9, are markedly enhanced in proliferated keratinocytes in CA lesions compared with normal skin, which is likely to be associated with anti-viral immunity triggered by HPV infection.

Key words: Toll-like receptor, HPV, Condyloma Acuminatum