中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (4): 278-280.

• 短篇论著 • 上一篇    下一篇

窄谱中波紫外线对人脐静脉内皮细胞的作用机制研究

李福民1,陆洁2,段西凌3,杨季云2   

  1. 1. 四川省医院皮肤科
    2.
    3. 四川省医学科学院四川省人民医院皮肤科
  • 收稿日期:2009-06-23 修回日期:2009-12-15 出版日期:2010-04-15 发布日期:2010-04-07
  • 通讯作者: 李福民 E-mail:fumin74@sina.com

Action mechanism of narrow-band UVB on human umbilical vein endothelial cells

  • Received:2009-06-23 Revised:2009-12-15 Online:2010-04-15 Published:2010-04-07
  • Contact: li fumin E-mail:fumin74@sina.com

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摘要：

目的：研究窄谱中波紫外线 (NB-UVB) 照射对血管内皮细胞的生物活性及相关细胞因子的作用和影响，进一步探讨窄谱中波紫外线对血管内皮细胞的作用机制。方法：人脐静脉内皮细胞（HUVEC）分别用50、100、200、300mJ/cm2NB-UVB照射，24小时后进行形态学观察、MTT法检测和 ELISA法检测MMP-1、MMP-2、VEGF含量。结果：细胞在不同剂量NB-UVB照射24小时后出现不同程度的肿胀变形、碎片增多，随剂量减低，损伤变化依次减轻；用MTT法检测显示细胞增殖活性出现明显下降，呈剂量依赖性； VEGF和MMP-1在NB-UVB照射后明显降低；除了300mJ/cm2 照射组其他照射组MMP-2与对照组比较无统计学差异。结论：NB-UVB照射可抑制HUVEC细胞活性和增殖率，呈剂量依赖性。NB-UVB对细胞分泌VEGF和MMP-1有抑制作用，对MMP-2的影响不明显。

关键词: 窄谱中波紫外线, 人脐静脉内皮细胞, MTT法, 基质金属蛋白酶, 血管内皮生长因子

Abstract:

Objective: To investigate the biological activity and the related cytokines of vascular endothelial cells treated with the narrow-band ultraviolet B(NB-UVB), and to evaluate the mechanism of action of NB-UVB on the vascular endothelial cells. Methods: The cells of human umbililcal vein endothelial cells (HUVEC) were incubated for 24 hours after the cells were treated with different dosage (50、100、200、300mJ/cm2) of NB-UVB radiation.Cell proliferation rates were determined by the MTT assay. The VEGF、MMP-1 and MMP-2 were determined by ELISA. Results: Morphological damage to different degrees was observed in all groups, including cytomorphosis, hydropic degeneration and the increase of cell debris. The changes was dependent on the dose of NB-UVB. The results of MTT assay showed that a significant decrease was observed, and the changes were dose-dependent. The secretion of VEGF and MMP-1 was significantly decreased. The secretion of MMP-2 was no difference except the group of 300mJ/cm2. Conclusion:.NB-UVB treatment caused a stable decrease of cell proliferation rates, and the changes were dose-dependent. NB-UVB treatment can inhibit the secretion of VEGF and MMP-1, but MMP-2.

Key words: Narrow-band Ultraviolet B, Human Umbilical Vein Endothelial Cell, MTT assay, MMP, VEGF