中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (10): 716-717.

• 短篇论著 • 上一篇    下一篇

多发性肌炎/皮肌炎患者外周血单一核细胞热休克蛋白90α，βmRNA的表达

刘继峰1,金海生2,徐田红2,李永伟2,许爱娥2   

  1. 1. 杭州市第三人民医院
    2. 杭州市第三人民医院皮肤科
  • 收稿日期:2008-09-24 修回日期:2008-11-20 发布日期:2009-10-09
  • 通讯作者: 刘继峰 E-mail:fliu8166@126.com
  • 基金资助:

    市(厅)级基金（编号）

mRNA expressions of heat shock protein 90 α, β in peripheral blood mononuclear cells of patients with polymyositis or dermatomyositis

  • Received:2008-09-24 Revised:2008-11-20 Published:2009-10-09
  • About author:刘继峰，男，38岁，山东费县人，副主任医师，医学博士，主要从事自身免疫性疾病和性传播疾病的研究。

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摘要：

目的 探讨多发性肌炎/皮肌炎（PM/DM）患者外周血单个核细胞（PBMC）中热休克蛋白90（HSP90）α，βmRNA表达与PM/DM患者对糖皮质激素（GC）疗效的相关性。方法 采用逆转录-聚合酶链反应（RT-PCR）方法检测29例GC治疗敏感、15例GC治疗抵抗的PM/DM患者及25例正常对照PBMC中HSP90 α，βmRNA的表达。结果 GC治疗敏感和抵抗的PM/DM患者PBMC中HSP90 α，βmRNA的表达均显著高于正常对照，且GC治疗抵抗的PM/DM患者PBMC中HSP90，βmRNA的表达显著高于GC治疗敏感的PM/DM患者。结论 HSP90α，βmRNA 表达水平升高可能与PM/DM患者的GC治疗抵抗相关。

Abstract:

Objective To explore the relationship between expressions of heat shock protein90(HSP90) α,βmRNA in peripheral blood mononuclear cell (PBMC) of patients with polymyositis/dermatomyositis(PM/DM) and the efficacy of glucocorticoid(GC) therapy.Methods Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of HSP90) α,βin PBMC of 29 cases of PM/DM patients sensitive to GC,15 cases of PM/DM patients resistant to GC and 25 cases of the normal control. Results The expressions of α,βmRNA in PBMC both of PM/DM patients sensitive to GC and resistant to GC were significantly higher than that of the normal control, and the expressions of HSP90α,βmRNA of PM/DM patients resistant to GC was significantly higher than that of PM/DM patients sensitive to GC. Conclusion The higher expression of HSP90α,βmRNA in PBMC of PM/DM patients may be correlated with PM/DM resistant to GC therapy.