中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (9): 616-618.

• 论著 • 上一篇    下一篇

Kaposi肉瘤组织内人类疱疹病毒8型K1基因型的初步分析

王晓东1,王星2,王慧2,等3   

  1. 1. 新疆医科大学第一附属医院皮肤科
    2. 新疆医科大学第一附属医院医学研究中心
    3. 湖北省中山医院整形美容外科
  • 收稿日期:2008-09-01 修回日期:2008-11-27 出版日期:2009-09-15 发布日期:2012-03-21
  • 通讯作者: 王晓东 E-mail:zhengdong18@sina.com
  • 基金资助:
    新疆医科大学第一附属医院科研奖励基金（2008-YFY-13）;无

Genotyping of human herpesvirus-8 K1 gene in Kaposi′s sarcoma

  • Received:2008-09-01 Revised:2008-11-27 Online:2009-09-15 Published:2012-03-21
  • Contact: 王晓东 WANG Xiao-Dong E-mail:zhengdong18@sina.com

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摘要： 目的 探讨新疆Kaposi肉瘤患者中人类疱疹病毒8型 （HHV-8）基因型特征及分布情况。方法 对11例本地Kaposi肉瘤冰冻标本进行组织基因组DNA抽提，应用巢氏PCR扩增K1基因。双向测序后绘制系统进化树，确定HHV-8基因型和分布特征。结果 11例本地的Kaposi肉瘤标本中A、C基因型分别为3和8例，其中A5和C7亚型是国内首次分离出。基因型分布呈成簇聚集和来源一致的特点。AIDS相关型和经典型Kaposi肉瘤HHV-8基因型一致。结论 新疆Kaposi肉瘤患者HHV-8基因型以A和C为主，并出现A5和C7亚型，与非洲和欧洲来源毒株的同源性很高。

关键词: 肉瘤，卡波西, 基因型, 疱疹病毒8型,人

Abstract: Objective To profile the genotype of human herpesvirus 8 （HHV-8） in patients with Kaposi′s sarcoma from Xinjiang Uygur Autonomous Region. Methods Genomic DNA was extracted from 11 frozen tissue samples of Kaposi′s sarcoma. The HHV-8 K1 gene was amplified by nested-PCR and sequenced. Phylogenetic tree was drawn by Mega 3.0 software. Results Of the 11 tissue samples, 3 were positive for A subtype of HHV-8 gene, 8 for C subtype. A5 and C7 subtypes were first isolated and identified in China. HHV-8 genotypes in these samples tended to cluster and share common origin. AIDS-Kaposi′s sarcoma-associated HHV-8 could share common genotypes with that associated with classic Kaposi′s sarcoma. Conclusions A and C predominate in the genotypes of HHV-8 in patients with Kaposi′s sarcoma in Xingjiang, which have a high homology with those strains from Africa and Europe. A5 and C7 genotypes of HHV-8 have been first isolated in China.

Key words: Human herpesvirus 8

中图分类号: 

  • R752.1