中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (8): 572-574.

• 论著 • 上一篇    下一篇

大麻素2型受体在皮肤恶性黑素瘤中的表达

赵梓纲1,2,杨洁3,李园园3,等4   

  1. 1. 解放军第306医院皮肤科
    2. 解放军总医院
    3. 北京 解放军总医院皮肤科
    4. 湖北省中山医院整形美容外科
  • 收稿日期:2008-07-07 修回日期:2009-02-28 出版日期:2009-08-15 发布日期:2009-08-10
  • 通讯作者: 赵梓纲

Expression and significance of cannabinoid receptor 2 in malignant melanoma

  • Received:2008-07-07 Revised:2009-02-28 Online:2009-08-15 Published:2009-08-10

PDF (PC)

335

赞

摘要：

目的 探讨大麻素2型受体（CB2受体）在色素痣及皮肤恶性黑素瘤中的表达规律及其意义。方法 免疫组化、RT-PCR检测色素痣和恶性黑素瘤组织中CB2受体在蛋白和mRNA不同水平的表达情况。结果 CB2受体在色素痣和恶性黑素瘤均有表达；在色素痣主要表达分布于痣细胞和表皮层的基底细胞层，在皮下组织中表达不明显；皮肤恶性黑素瘤与色素痣表达CB2的强度间差异有统计学意义（P < 0.05）。结论 恶性黑素瘤中CB2受体在蛋白和基因水平表达均升高，恶性黑素瘤CB2受体表达强度明显大于色素痣。

关键词: 大麻素受体;皮肤肿瘤;免疫组织化学;RT-PCR

Abstract:

Objective To investigate the expression of cannabinoid receptor 2 （CB2） in pigmented nevus and malignant melanoma and its significance. Methods The protein and mRNA expressions of CB2 were measured in tissue samples of 20 patients with pigmented nevus and 20 patients with malignant melanoma using immunohistochemical staining and RT-PCR, respectively. Results CB2 was expressed in both pigmented nevus and malignant melanoma. In tissue of pigmented nevus, CB2 was chiefly expressed in nevocytes and basal cell layer, and only a small quantity of CB2 was expressed in subcutaneous tissue. Statistical differences were observed between samples of malignant melanoma and pigmented nevus in the expression intensity of CB2（P < 0.05）. Conclusions Increased protein and mRNA expressions of CB2 are observed in tissue of malignant melanoma, and the expression intensity of CB2 is higher in tissue of malignant melanoma than in that of pigmented nevus.