Abstract:

Abstract：Objective: To explore the inhibition of the nm-HAP-sol to the generation of Human Skin Squamous Cell Carcinoma Cell (A431 cell). Methods: Nm-HAP-sol was prepared by transonic- emulsification. A431 cells were cultured with DMEM and the corresponding concentration of nm-HAP-sol in the 400mg/L，200mg/L，100mg/L，50mg/L nm-HAP-sol groups. At the 1, 2, 3, 4, 5 days, calculating cell inhibition ratio by MTT colorimetric and observing the the change of cell morphosis by inverted microscope. A431 cells were cultured with DMEM and 10µg/ml 5-Fu in the positive control group. Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%, (77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope. Conclusion: The nm-HAP-sol has a very evident inhibition to the generation of A431 cells in a time and dose dependent way.