中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (6): 421-424.

• 论著 • 上一篇    下一篇

青蒿素、青蒿琥酯抗皮肤瘢痕的研究

农晓琳1,陈洪1,陈石海2,李佳荃3,李菊裳4,韦高5,邓凌6,李昊7   

  1. 1. 广西医科大学口腔医学院
    2. 南宁市广西医科大学第一附属医院整形美容外科
    3. 广西医科大学医学实验中心
    4. 南宁广西医科大学一附院皮肤科
    5. 南宁市广西医科大学一附院皮肤科
    6. 广西医科大学， 口腔医学院口腔颌面外科
    7. 广西医科大学，口腔医学院口腔颌面外科
  • 收稿日期:2008-10-29 修回日期:2008-12-10 出版日期:2009-06-15 发布日期:2009-06-03
  • 通讯作者: 农晓琳
  • 基金资助:

    广西自然科学基金资助项目（桂科青0447033);省级基金（编号）

Arteannuin and Artesunate Ointment on Inhibiting and Curing Rabbit Ear Hypertrophic Scar Model

  • Received:2008-10-29 Revised:2008-12-10 Online:2009-06-15 Published:2009-06-03
  • Contact: Nong Xiaolin

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摘要：

目的 检测青蒿素和青蒿琥酯对兔耳增生性瘢痕模型瘢痕防治的效果，探索建立抗皮肤瘢痕膏剂的有效配方。方法 采用青蒿素和青蒿琥酯配制外用膏剂治疗兔耳腹侧增生性瘢痕模型，用药28天后切取瘢痕HE染色、VG染色，检测瘢痕组织的增生指数（Hypertrophic Index，HI）、成纤维细胞数密度（Numerical density on area，NA）及胶原纤维面积密度（Area density on area，AA）。结果 青蒿素、青蒿琥酯配制的膏剂组均较对照组瘢痕平坦、柔软且成纤维细胞胞体变小，胶原纤维排列较稀疏、整齐。青蒿素组HI、NA、AA分别为(1.452±0.27)、(3638.245±463.0)细胞/mm2、(32.29±6.9) %;青蒿琥酯组HI、NA、AA分别为(1.445±0.24)、(3585.016±638.9)细胞/mm2、(34.74±8.27) %。两实验组检测指标均小于基质对照组及空白对照组，p<0.01。青蒿素组与青蒿琥酯组相比较，p >0.05，无统计学差异。结论 青蒿素、青蒿琥酯膏可有效地抑制和治疗动物实验性皮肤瘢痕，提示其可作为抗皮肤瘢痕外用制剂的成份药。

关键词: 青蒿素;青蒿琥酯;皮肤;瘢痕;动物模型

Abstract:

Objective Investigate the effects of Artemisinin and artesunate on animal hypertrophic scar tissues. Methods Full-thickness wounds to cartilage were created in New Zealand white rabbit ears. Artemisinin or artesunate cream treatments were applied to the hypertrophic scars grew in ventral side of rabbit ears, 28 days after the wound created. 28 days after the treatment, the animals were scarify, scars were harvested incision, and examined under HE-staining ，VG-staining, by hypertrophy index (HI), numerical density of fibroblasts on area (NA) and area density of collagen fibers on area (AA) detected. Results Compared with control groups, the Artemisinin and artesunate ointment treated scars groups were softer and flatter; the fibroblasts grew downward and the collagen fibers appeared to be regulated and sparcer. HI, NA, AA in Artemisinin group were (1.452±0.27), (3638.245±463.0)cell/mm2, (32.29±6.9) %; in artesunate group were (1.445±0.24), (3585.016±638.9)cell/mm2, (34.74±8.27) % respectly. Artemisinin and artesunate groups were significantly differences with untreated control and medium control in HI，NA and AA, p <0.01. No significant different was found between Artemisinin and artesunate group, p>0.05. Conclusion Artemisinin and artesunate ointment have reliable effect on inhibiting and curing animal skin scar hyperplasia．Artemisinin and artesunate might have potency to be component of scar prevent and treatment ointment.

Key words: Artemisinin;Artesunate;Skin;scar;Model, animal