中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (5): 305-307.

• 论著 • 上一篇    下一篇

温热对HPV-6/11型早期基因E6和E7表达的影响

祝立丽1,高兴华1,李晓东1,王雅坤1,王晓琴2,陈洪铎3   

  1. 1. 沈阳 中国医科大学附属第一医院皮肤科
    2. 沈阳 中国医科大学附属第二医院皮肤科
    3. 沈阳 中国医科大学第一附属医院皮肤科,No.1 Hospital of China Medical University
  • 收稿日期:2008-08-19 修回日期:2008-10-27 出版日期:2009-05-15 发布日期:2009-05-13
  • 通讯作者: 祝立丽
  • 基金资助:

    国家自然科学研究基金（30740082）；教育部创新团队支持计划项目（IRT0760）

Effects of hyperthermia on the expression of E6 and E7 genes of human papillomavirus type 6 and 11

ZHU Li-Li , , ,, , ,CHEN Hong-Duo   

  • Received:2008-08-19 Revised:2008-10-27 Online:2009-05-15 Published:2009-05-13
  • Contact: ZHU Li-Li

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摘要：

目的 探讨不同温热条件对HPV感染皮损中E6/E7基因表达的影响。方法 采用PCR法确定6例尖锐湿疣标本HPV型别。利用37 ℃，42 ℃，45 ℃不同的温热条件处理置于培养液中的离体尖锐湿疣皮损，采用实时定量PCR法检测HPV-6/11型早期基因E6/E7 mRNA的表达水平。结果 6例标本中，各有1例分别感染HPV-6、HPV-11，4例同时感染HPV-6和HPV-11。E6、E7 mRNA的表达水平均随温度升高而逐渐减少。HPV-6 E6 mRNA的相对表达量分别为37 ℃（1.00 ± 0.00），42 ℃（0.61 ± 0.17），45 ℃（0.27 ± 0.15）；HPV-6 E7 mRNA的相对表达量分别为37 ℃（1.00 ± 0.00），42 ℃（0.56 ± 0.21），45 ℃（0.16 ± 0.11）；HPV-11 E6 mRNA的相对表达量分别为37 ℃（1.00 ± 0.00），42 ℃（0.60 ± 0.22），45 ℃（0.16 ± 0.08）；HPV-11 E7 mRNA相对表达量分别为37 ℃（1.00 ± 0.00），42 ℃（0.55 ± 0.15），45 ℃（0.24 ± 0.06）。组间比较差异有统计学意义（P < 0.01）。结论 温热对HPV-6/11型E6和E7基因表达的抑制作用可能是感染消退的机制之一。

关键词: 局部温热;E6/E7基因;实时定量PCR

Abstract:

Objective To investigate the effect of hyperthermia on the expression of E6 and E7 genes of human papillomavirus （HPV） type 6 and 11 in HPV-infected human skin. Methods Tissue samples were obtained from the lesions of condyloma accuminatum （CA） in 6 patients after informed consent. Each sample was divided into 4 parts: one was embedded and directly stored at -80 ℃; the other 3 parts were placed in culture medium and the surface of the samples was irradiated for 30 minutes with a thermotherapy apparatus at 37 ℃, 42 ℃, 45 ℃, respectively, then the samples were taken out and stored at -80 ℃. RNA was extracted from the specimens, real time quantitative PCR （qPCR） was performed to detect the expression of E6 and E7 genes of HPV-6 and -11. Results Of the 6 patients, 2 were infected with HPV-6 and -11 respectively, 4 with both HPV-6 and HPV-11. The expression of E6 and E7 mRNA decreased with the increase in irradiation temperature. The relative mRNA expression levels at 37 ℃, 42 ℃ and 45 ℃ were 1.00 ± 0.00, 0.61 ± 0.17, 0.27 ± 0.15, respectively, for HPV-6 E6 gene, 1.00 ± 0.00, 0.56 ± 0.21, 0.16 ± 0.11 respectively, for HPV-6 E7 gene, 1.00 ± 0.00, 0.60 ± 0.22, 0.16 ± 0.08, respectively, for HPV-11 E6 gene, 1.00 ± 0.00, 0.55 ± 0.15, 0.24 ± 0.06, respectively, for HPV-11 E7 gene; statistical difference was noted among them between the specimens irradiated at different temperature （all P < 0.01）. Conclusion Hyperthermia can remarkably suppress the expression of HPV-6/11 E6 and E7 genes, which may be a possible mechanism under the regression of warts induced by local hyperthermia.