中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (4): 259-261.

• 论著 • 上一篇    下一篇

SLE患者外周血单一核细胞淋巴细胞功能相关抗原1表达的检测

张耀华1,施伟民2,徐金华1   

  1. 1. 复旦大学附属华山医院皮肤科
    2. 上海同济大学附属同济医院皮肤科
  • 收稿日期:2008-05-12 修回日期:2008-07-30 出版日期:2009-04-15 发布日期:2009-04-14
  • 通讯作者: 徐金华，xjhhuashan@yahoo.com.cn
  • 基金资助:

    市(厅)级基金（编号）

Detection of lymphocyte function-associated antigen-1 on peripheral blood mononuclear cells from patients with systemic lupus erythematosus

  • Received:2008-05-12 Revised:2008-07-30 Online:2009-04-15 Published:2009-04-14

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摘要：

目的 研究SLE患者外周血单一核细胞（PBMC）淋巴细胞功能相关抗原1（LFA-1）的表达，探讨LFA-1与SLE的发病及病情变化的关系及其临床意义。方法 提取30例SLE患者（SLE组）和24例正常人（对照组）的PBMC，用抗人CD3-FITC和抗人CD11a-PE对其进行标记后，采用流式细胞术检测其CD11a的表达率。结果 PBMC CD11a的表达率对照组为68.21% ± 4.58%，SLE组为73.74% ± 7.89%，活动期组为77.11% ± 7.46%，稳定期组为69.33% ± 6.27%，SLE组PBMC CD11a的表达率显著高于对照组（P ＜ 0.05），且活动期组亦显著高于对照组（P ＜ 0.05），而稳定期组与对照组比较，差异无统计学意义（P > 0.05）。活动期组与稳定期组比较，差异有统计学意义（P ＜ 0.05）。相关性分析显示，SLE患者CD11a的表达率与其采血时的SLEDAI呈正相关（r = 0.64，P ＜ 0.01）。结论 LFA-1的过表达参与SLE的发病，并与疾病活动相关。

关键词: 系统性红斑狼疮;淋巴细胞功能相关抗原-1;CD11a

Abstract:

Objective To investigate the expression of lymphocyte function-associated antigen-1 （LFA-1） on peripheral blood mononuclear cells（PBMCs） from patients with SLE and its relation to the occurrence and development of SLE. Methods PBMCs were obtained from 30 patients with SLE and 24 normal human controls. After labeled with anti-CD3-FITC and anti-CD11a-PE, PBMCs were detected for the expression of CD11a by flow cytometric analysis. Results The positivity rate of CD11a was significantly higher in patients with SLE and those with active SLE than in the normal controls （73.74% ± 7.89% and 77.11% ± 7.46% vs 68.21% ± 4.58%, both P < 0.05）. Furthermore, increased positivity rate of CD11a was observed in patients with active SLE compared with those with stable SLE （77.11% ± 7.46% vs 69.33% ± 6.27%, P < 0.05）, but there was no significant difference between the latter group and normal controls （P > 0.05）. In addition, the expression level of CD11a was positively correlated with SLE disease activity index （r = 0.64, P < 0.01） in patients. Conclusion The overexpression of LFA-1 may take part in the occurrence of SLE and correlate with the activity of SLE.

中图分类号: 

  • R 571