中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (1): 42-44.

• 论著 • 上一篇    下一篇

COX-2抑制剂NS398对人鳞状细胞癌Tca8113细胞株生长和凋亡的影响

曾维惠 郑焱 耿松梅 袁景奕 牛新武 谭升顺   

  1. 西安交通大学医学院附属第二医院 西安交通大学第二医院皮肤科 西安交通大学第二附属医院皮肤科 西安交通大学第二医院皮肤科 西安市西安交通大学第二医院皮肤科 西安交通大学第二医院皮肤科
  • 收稿日期:2008-01-14 修回日期:2008-07-01 出版日期:2009-01-15 发布日期:2009-01-15
  • 通讯作者: 曾维惠 E-mail:zengweihui@medmail.com.cn

Effects of NS398, an inhibitor of cyclooxygenase 2, on the growth and apoptosis of human squamous cell carcinoma cell line Tca8113

Zeng Wei-Hui   

  • Received:2008-01-14 Revised:2008-07-01 Online:2009-01-15 Published:2009-01-15
  • Contact: Zeng Wei-Hui E-mail:zengweihui@medmail.com.cn

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摘要：

目的 探讨COX-2抑制剂NS398对鳞状细胞癌细胞Tca8113生长及凋亡的影响。方法 细胞培养加入NS398作用后，采用噻唑蓝（MTT） 法观察NS398 对Tca8113细胞增殖的影响，流式细胞仪及透射电镜研究NS398对Tca8113细胞周期和凋亡的作用。结果 MTT比色法显示NS398能抑制Tca8113细胞的生长，呈浓度和时间依赖性。流式细胞仪检测结果显示NS398干预细胞出现典型的亚二倍体"凋亡峰"；G0 /G1期细胞比例升高，S期和G2 /M期比例下降。电镜下见典型的凋亡形态学改变。结论COX-2抑制剂NS398在体外可通过诱导凋亡有效抑制Tca8113细胞的生长。

关键词: 鳞状细胞癌；COX-2；TCa8113； NS398

Abstract:

Objective To study the effect of NS398, an inhibitor of cyclooxygenase 2 （COX2）, on the growth and apoptosis of human squamous cell carcinoma cell line Tca8113. Methods Cultured Tca8113 cells were incubated with NS398 （0, 6.25, 12.5, 25, 50, 100 μmol/L） for 24, 48 and 72 hours, respectively. Thereafter, MTT method, flow cytometry and transmission electron microscopy were applied to detect the proliferation, cell cycle and apoptosis of Tca8113 cells, respectively. Results The proliferation of Tca8113 cells was inhibited by NS398 in a dose- and time-dependent manner （both P < 0.05）. FCM analysis showed the appearance of a typical hypodiploid apoptotic （Sub-G1） peak, an increase in the percentage of cells at G0/G1 phase and a decrease in that at S and G2/M phases in NS398 （100 μmol/L） -treated Tca8113 cells. Moreover, the cell proliferation index was significantly downregulated by NS398 of 100 μmol/L from 41.03 to 24.33 （P < 0.05）. Under an electron microscope, morphological changes characteristic of apoptosis were observed in NS398-treated Tca8113 cells. Conclusion NS398, an inhibitor of COX2, could effectively inhibit the growth of Tca8113 cells in vitro by induction of apoptosis.

Key words: Key word: SSC; TCa8113; COX-2; NS398;