中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (1): 8-11.

• 论著 • 上一篇    下一篇

不同剂量UVA1辐射硬皮病鼠模型的研究

鞠梅 张青松 张小华 陈昆 常宝珠 顾恒   

  1. 南京医科院皮研所 南京 中国医学科学院皮肤病研究所 南京医科院皮研所 南京医科院皮研所 南京医科院皮研所 南京医科院皮研所
  • 收稿日期:2008-04-07 修回日期:2008-04-23 出版日期:2009-01-15 发布日期:2009-01-15
  • 通讯作者: 鞠梅 E-mail:jumeiweng@163.com

Experimental study on the effect of different doses of UVA1 irradiation in the treatment of mouse model of scleroderma

  • Received:2008-04-07 Revised:2008-04-23 Online:2009-01-15 Published:2009-01-15

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摘要：

目的 探讨不同剂量的UVA1辐射硬皮病鼠模型后皮肤厚度和胶原含量的变化。方法 用100 μL（400 μg/mL）的博来霉素皮下注射BALB/c小鼠背部，共4周，建立硬皮病的小鼠模型，然后将小鼠随机分为空白对照组、模型对照组、UVA1照射组（100 J/cm2、60 J/cm2、20 J/cm2）及阴性对照组。每周照射3次，共10周，观察并比较各组小鼠皮肤组织病理改变、皮肤厚度和胶原含量的变化。结果 模型对照组与空白对照组相比，皮肤厚度（t = 4.945，P < 0.001）和胶原含量（t = 3.712，P < 0.01）的差异均有统计学意义。UVA1照射组小鼠皮肤变软、变薄，但只有HD-UVA1组小鼠皮肤厚度、胶原含量与模型对照组、阴性对照组比较，差异有统计学意义（P < 0.05）。照射组之间相互比较，皮肤厚度差异F = 14.853，P < 0.01，胶原含量差异F = 6.317，P < 0.01，尤其是HD-UVA1与MD-UVA1和LD-UVA1之间有显著差异。结论 HD-UVA1照射能明显改善博来霉素所致的硬皮病鼠模型的皮肤厚度、胶原含量的变化，可能与其抑制皮肤的胶原增生、降低胶原含量有关。

关键词: UVA1;硬皮病;鼠模型;实验研究

Abstract:

Objective To compare the changes of skin thickness and collagen content in mouse models of scleroderma after irradiated with different doses of UVA1, so as to seek the suitable irradiation dose in the treatment for scleroderma. Methods Sixty mice were randomly and equally divided into 6 groups: blank control group （no injection and no irradiation）, model control group （injected only and killed 2 days after the last injection）, high-dose （HD） UVA1 group （injected with bleomycin and irradiated with UVA1 of 100 J/cm2）, medium-dose （MD） UVA1 group （injected with bleomycin and irradiated with UVA1 of 60 J/cm2）, low-dose （LD） UVA1 group （injected with bleomycin and irradiated with UVA1 of 20 J/cm2）, and negative control group （injected only and killed until the end of irradiation）. Experimental mouse models of scleroderma were established by subcutaneous injection of 100 μL bleomycin （BLM） at 400 μg/mL into the back of BALB/c mouse once a day for 4 weeks. Phototherapy was performed 3 times weekly for 10 weeks. Thereafter, skin specimens were obtained from the injected or irradiated back of mice, and subjected to an observation on pathological changes of skin tissue and thickness, as well as the measurement of collagen content. Results There was statistical differences between blank control group and model control group in both the skin thickness （t = 4.945, P < 0.001） and collagen content （t = 3.712, P < 0.01). UVA1 phototherapy improved the skin sclerosis and reduced the thickness in mouse models, but significant effect was only observed with HD-UVA1 in both the parameters（both P < 0.05）. There was significant difference among the 3 groups receiving phototherapy in the changes of skin thickness （F = 14.853, P < 0.01） and collagen content （F = 6.317, P < 0.01), and HD-UVA1 was significantly more effective than MD-UVA1 and LD- UVA1. Conclusion High-dose UVA1 irradiation could significantly reduce the changes in skin thickness and collagen content in mouse model of sclerosis induced by bleomycin，which may be related to its inhibition on collagen fiber proliferation and decrease in collagen content.

Key words: UVA1;scleroderma;mice model;experimental study