中华皮肤科杂志

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γ-干扰素、全反式维A酸对皮肤鳞状细胞癌SCL-1细胞表达生存素的影响

王珍, 何春涤, 金鑫, 肖汀, 王雅坤, 金光玉, 陈江, 姜奕, 陈洪铎   

  1. 中国医科大学附属第一医院皮肤科, 沈阳, 110001
  • 收稿日期:2006-10-20 出版日期:2007-06-15 发布日期:2007-06-15
  • 通讯作者: 何春涤,email:chundihe@hotmail.com E-mail:chundihe@hotmail.com
  • 基金资助:
    国家自然科学基金(30271198);教育部留学回国人员科研启动基金[2002]247号

Effects of all-trans-retinoic acid andγ-interferon on the expression of survivin in SCL-1 cells

WANG Zhen, HE Chun-di, JIN Xin, XIAO Ting, WANG Ya-kun, JING Guang-yu, CHEN Jiang, JIANG Yi, CHEN Hong-Duo   

  1. Department of Dermatology, No.1 Hospital of China Medical University, Shenyang 110001, China
  • Received:2006-10-20 Online:2007-06-15 Published:2007-06-15

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摘要： 目的 探讨γ-干扰素、全反式维A酸(ATRA)对皮肤鳞状细胞癌细胞株(SCL-1)表达生存素的影响。方法 采用RT-PCR、免疫印迹(Western blot)分析方法分别检测γ-IFN、ATRA作用前后SCL-1细胞株生存素mRNA及蛋白表达的差异。结果 γ-IFN处理后,生存素在SCL-1细胞表达较对照组明显下降(P<0.05),且具有时间依赖性和剂量依赖性;ATRA可以增强γ-IFN对生存素的抑制作用。结论 γ-IFN联合ATRA诱导皮肤鳞状细胞癌细胞发生细胞凋亡的机制之一可能与下调生存素的表达相关。

关键词: 癌, 鳞状细胞, 维生素A酸类, 干扰素Ⅱ型, 生存素, 细胞系, 肿瘤

Abstract: Objective To study the effects of all-trans-retinoic acid(ATRA)andγ-interferon(γ-IFN)on the expression of survivin in skin squamous cell carcinoma cell line SCL-1.Methods SCL-1 cells were treated withγ-IFN alone(100,500,1000 u/mL,respectively)or in combination with ATRA(1000 u/mLγ-IFN+10-6mol/mL ATRA)in vitro.The mRNA and protein expressions of survivin were detected in SCL-1 cells by RT-PCR and Western-blot respectively.Result sγ-IFN could significantly inhibit the mRNA and protein expressions of survivin in a dose-and time-dependent manner.Also,ATRA could enhance the inhibition of survivin expression byγ-IFN.Conclusion The down-regulation of survivin ex-pression may be a mechanism for the induction of apoptosis of skin squamous cell carcinoma cells by the combination ofγ-IFN and ATRA.

Key words: Carcinoma, squamous cell, Retinoids, Interferon typeⅡ, Survivin, Cell line, tumor