中华皮肤科杂志

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CpG序列促进马疫锥虫kDNA诱生IgG型抗dsDNA抗体

夏育民1, 徐世正1, 丁国华2, 江珊1, 熊蜡元2   

  1. 1. 武汉大学人民医院皮肤科, 430060;
    2. 武汉大学人民医院肾病内科, 430060
  • 收稿日期:2006-06-30 出版日期:2007-06-15 发布日期:2007-06-15

CpG containing oligodeoxynucleotides enhance the induction of IgG subtype of anti-dsDNA antibodies by kinetoplast DNA derived from Trypanosoma equiperdum

XIA Yu-min1, XU Shi-zheng1, DING Guo-hua2, JIANG Shan1, XIONG La-yuan2   

  1. Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2006-06-30 Online:2007-06-15 Published:2007-06-15

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摘要： 目的 探讨CpG寡核苷酸序列参与马疫锥虫动基体DNA(kDNA)诱生IgG型抗双链DNA(dsDNA)抗体过程中的作用。方法 分别以CpG寡核苷酸序列、不完全弗氏佐剂(IFA)或两者混合物作为佐剂,将纯化的马疫锥虫kDNA通过皮下注射途径免疫正常的BALB/c小鼠。免疫结束后,检测其血清中抗dsDNA抗体等指标。结果 未添加免疫佐剂的马疫锥虫kDNA仅能诱生IgM型抗dsDNA抗体,而添加CpG序列或IFA后皆能诱生IgG型抗dsDNA抗体,但CpG序列诱生的IgG型抗体水平更高(P<0.05)。结论 CpG序列能更有效地促进马疫锥虫kDNA诱生IgG型抗dsDNA抗体。

关键词: 红斑狼疮, 系统性, 抗核抗体, 寡核苷酸序列分析, 锥虫属

Abstract: Objective To study the role of CpG containing oligodeoxynucleotide(ODN)in the in-duction of IgG subtype of anti-dsDNA antibodies by kinetoplast DNA(kDNA)derived from Try-panosoma equiperdum(TE).Methods kDNA was extracted from TE and purified,then was injected sub-cutaneously into immunize normal BALB/c mice,with CpG ODN,incomplete Freund's adjuvant(IFA),or a mixture of CpG ODN and IFA as immune adjuvant.The serum titers of anti-dsDNA antibodies were deter-mined with TE as the substrate by ELISA after immunization.The levels of total IgG,C3,IL-10 and IFN-γwere also detected by single immunodiffusion test and ELISA respectively.Results Without any immune adjuvant,TE kDNA alone could only induce IgM subtype of anti-dsDNA antibodies,while CpG ODN or I-FA induced the IgG subtype of anti-dsDNA antibodies.CpG ODN had a stronger capability to enhance the induction of anti-dsDNA antibodies by kDNA than IFA(P<0.05).Conclusion CpG ODN can enhance the induction of IgG subtype of anti-dsDNA antibodies by TE kDNA efficiently.

Key words: Lupus erythematosus, systemic, Antinuclear antibodies, Oligonucleotide array sequence analysis, Trypanosoma