中华皮肤科杂志

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308 nm准分子光对人角质形成细胞的凋亡、细胞周期和干细胞因子表达的影响

张勇, 项蕾红, 李剑, 祝绿川, 郑志忠   

  1. 复旦大学附属华山医院皮肤科, 上海, 200040
  • 收稿日期:2006-08-14 出版日期:2007-06-15 发布日期:2007-06-15
  • 通讯作者: 项蕾红,email:xlh@medmail.com.cn E-mail:xlh@medmail.com.cn
  • 基金资助:
    国家自然科学基金(30471560)

Effect of 308 nm monochromatic excimer light on the apoptosis, cell cycle and stem cell factor expression of human keratinocytes

ZHANG Yong, XIANG Lei-hong, LI Jian, ZHU Lü-chuan, ZHENG Zhi-zhong   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2006-08-14 Online:2007-06-15 Published:2007-06-15

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摘要： 目的 探讨单频308 nm准分子光对角质形成细胞的凋亡、细胞周期和干细胞因子表达的影响。方法 采用MTT法确定最适能量密度,用流式细胞仪检测单频308 nm准分子光对角质形成细胞的凋亡和细胞周期的影响。RT-PCR和蛋白质印迹分别检测单频308nm准分子光对角质形成细胞干细胞因子基因转录和蛋白表达水平的影响。结果 用400 mJ/cm2单频308 nm准分子光照射角质形成细胞后24h和48h,细胞凋亡明显增加(P<0.05),48h时S期细胞虽有增加,但无统计学意义(P=0.059)。干细胞因子mRNA和蛋白表达均增加,在照射后12h左右达高峰。且蛋白表达随着照射剂量的增加而增加,但mRNA的表达未随剂量的增加而改变。结论 单频308 nm准分子光可导致角质形成细胞凋亡,促进干细胞因子的表达,这可能是准分子光治疗白癜风的机制之一。

关键词: 角蛋白细胞, 细胞凋亡, 干细胞因子, 准分子光

Abstract: Objective To investigate the effect of 308 nm monochromatic excimer light on the apoptosis,cell cycle and stem cell factor(SCF)expression of human keratinocytes.Methods MTT was used to determine the optimal dose of the excimer light on the keratinocytes,and flow cytometry to examine the apoptosis and cell cycle of keratinocytes.The mRNA and protein expression of SCF was detected by RT-PCR and Western blot,respectively.Results The apoptosis rate of keratinocytes was significantly increased at 24 h and 48 h after the radiation with 308 nm monochromatic excimer light of 400 mJ/cm2(both P<0.05).The cell count at S stage was elevated 48 h after the radiation,but no statistical difference was found for the elevation.Both the mRNA and protein expression of SCF increased after the radiation, and peaked at 12 h.The excimer light up-regulated the protein expression,but not mRNA expression of SCF,in a dose dependant manner.Conclusions The 308 nm excimer light could induce the apoptosis,and promote the expression of SCF mRNA and protein expression of keratinocytes,which might be a therapeutic mechanism of 308 nm excimer light in vitiligo.

Key words: Keratinocytes, Apoptosis, Stem cell factor, Excimer light