枸杞多糖对中波紫外线照射HaCaT细胞低氧诱导因子1α、血管内皮细胞生长因子表达的影响

中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (9): 667-670.

枸杞多糖对中波紫外线照射HaCaT细胞低氧诱导因子1α、血管内皮细胞生长因子表达的影响

张洪长¹,左立春²,孙海才²,律广富¹,李娜¹,林喆¹

1. 长春中医药大学
2. 长春中德骨科医院

收稿日期:2016-12-26 修回日期:2017-02-16 出版日期:2017-09-15 发布日期:2017-08-31
通讯作者: 林喆 E-mail:linzhe1228@163.com
基金资助:
吉林省科技厅科技发展计划项目

Effect of Lycium barbarum polysaccharide on of vascular endothelial growth factor and hypoxia inducible factor-1α in ultraviolet B-radiated HaCaT cells

hong-chang ZHANG¹,Li-chun ZUO², ³, ¹, ³,Zhe LIN

Received:2016-12-26 Revised:2017-02-16 Online:2017-09-15 Published:2017-08-31
Contact: Zhe LIN E-mail:linzhe1228@163.com

摘要/Abstract

摘要： 目的探讨枸杞多糖对中波紫外线（UVB）照射HaCaT细胞低氧诱导因子1α（HIF?1α）、血管内皮细胞生长因子（VEGF）的表达。方法将常规培养的HaCaT细胞，分为对照组和枸杞多糖给药组（12.5、25.0、50.0、100.0 μg/ml浓度），各组在给药4 h后同时进行不同强度UVB（0、20、40、60 mJ/cm2）照射，培养24 h。用CCK?8法检测细胞生存率。酶生化法检测细胞内超氧化物歧化酶（SOD）的活性；RT?PCR及Western 印迹检测HIF?1α、VEGF基因及蛋白的表达。结果对照组同照射剂量比，枸杞多糖（12.5 、25.0 、100.0 μg/ml浓度）各组不同程度提高UVB照射损伤细胞的生存率（P < 0.05），以50.0 μg/ml 枸杞多糖组升高最明显，差异有统计意义（P < 0.01）。枸杞多糖各给药组SOD活性以50.0 μg/ml SOD活性升高最明显，差异有统计学意义（P < 0.01）。随着UVB照射剂量（20、40、60 mJ/cm2）增加，HIF?1α、VEGF基因、蛋白的表达也逐渐增高，与对照组相比，50.0 μg/ml 枸杞多糖给药组能够有效降低细胞内HIF?1α、VEGF基因及蛋白的表达，差异有统计意义（P < 0.05）。结论枸杞多糖通过对HaCaT细胞内HIF?1α、VEGF基因及蛋白表达的影响，可能参与预防UVB照射损伤的过程。

Abstract: Zhang Hongchang, Zuo Lichun, Sun Haicai, Lyu Guangfu, Li Na, Lin Zhe Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China（Zhang HC, Lyu GF, Li N, Lin Z）; Changchun Sino-German Orthopaedic Hospital, Changchun 130051, China （Zuo LC, Sun HC） Corresponding author: Lin Zhe, Email: linzhe1228@163.com 【Abstract】 Objective To evaluate effects of Lycium barbarum polysaccharide （LBP） on of vascular endothelial growth factor （VEGF） and hypoxia inducible factor-1α（HIF-1α） in ultraviolet B （UVB）-radiated HaCaT cells. Methods Conventionally cultured HaCaT cells were divided into control group and LBP groups, which were firstly treated with DMEM, 12.5, 25.0, 50.0 and 100 μg/ml LBP solution respectively for 4 hours, and then were irradiated by UVB at different intensity of 0, 20, 40, 60 mJ/cm2 separately. After 24-hour continuing culture, CCK-8 assay was performed to determine the cell survival rate, and an enzymatic-biochemical method to estimate the activity of superoxide dismutase（SOD）. RT-PCR and Western blot analysis were conducted to measure the mRNA and protein of HIF-1α and VEGF respectively. Results Compared with the control group at the same UVB radiation dose, the 12.5-, 25.0- and 100.0-μg/ml LBP groups showed different extents of increase in survival rates of UVB-radiated cells （P < 0.05）, and the 50.0-μg/ml LBP group showed the highest cell survival rate （P < 0.01）. Among all the LBP groups, SOD activity was highest in the 50.0-μg/ml LBP group （P < 0.01）. Along with the increase of UVB radiation dose, the mRNA and protein of HIF-1α and VEGF all gradually increased. Compared with the control group, the 50.0-μg/ml LBP group could effectively reduce the mRNA and protein of HIF-1α and VEGF in HaCaT cells （all P < 0.05）. Conclusion LBP may play a role in protecting cells from UVB radiation-mediated damage, likely by influencing the mRNA and protein of HIF-1α and VEGF in HaCaT cells.

中图分类号:

R961

张洪长左立春孙海才律广富李娜林喆. 枸杞多糖对中波紫外线照射HaCaT细胞低氧诱导因子1α、血管内皮细胞生长因子表达的影响[J]. 中华皮肤科杂志, 2017, 50(9): 667-670.

hong-chang ZHANG Li-chun ZUO Zhe LIN. Effect of Lycium barbarum polysaccharide on of vascular endothelial growth factor and hypoxia inducible factor-1α in ultraviolet B-radiated HaCaT cells[J]. Chinese Journal of Dermatology, 2017, 50(9): 667-670.

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