白细胞介素22在玫瑰糠疹患者外周血单一核细胞及血清中的表达

中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (10): 746-747.

白细胞介素22在玫瑰糠疹患者外周血单一核细胞及血清中的表达

孙怡¹,王振华²

1. 潍坊市人民医院皮肤科
2. 山东省潍坊市人民医院皮肤科

收稿日期:2013-10-31 修回日期:2014-05-09 发布日期:2014-10-01
通讯作者: 王振华 E-mail:wfzcwzh@126.com

Expressions of interleukin-22 in sera and peripheral blood mononuclear cells of patients with pityriasis rosea

,zhenhua wang

Received:2013-10-31 Revised:2014-05-09 Published:2014-10-01
Contact: zhenhua wang E-mail:wfzcwzh@126.com

摘要/Abstract

摘要： 【摘要】目的探讨白细胞介素（IL）-22在玫瑰糠疹发病机制中的作用。方法荧光实时定量反转录（RT）-PCR检测玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22mRNA的表达水平。采用酶联免疫吸附试验检测血清中IL-22的水平。结果玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22 mRNA的相对表达量分别为4.15 ± 1.36、2.89 ± 0.91和1.91 ± 0.60，急性期明显高于恢复期（t = 3.61，P ＜ 0.05），恢复期高于健康对照组（t = 3.84，P ＜ 0.05）。玫瑰糠疹患者急性期、恢复期及健康对照组血清中IL-22水平分别为（36.87 ± 10.63）、（21.44 ± 7.33）和（13.22 ± 4.26） ng/L，急性期明显高于恢复期（t = 5.01，P ＜ 0.05），恢复期高于健康对照组（t = 4.49，P ＜ 0.05）。血清IL-22水平与玫瑰糠疹病程呈正相关（r = 0.883，P ＜ 0.05）。结论 IL-22在玫瑰糠疹患者外周血及血清中表达明显升高，可能在玫瑰糠疹的发病中起一定作用。

关键词: 糠疹，玫瑰, 白细胞介素22, 发病机制

Abstract: Sun Yi, Wang Zhenhua. Department of Dermatology, Weifang People′s Hospital, Weifang 261041, Shandong, China Corresponding author: Wang Zhenhua, Email: wfzcwzh@126.com 【Abstract】 Objective To investigate the role of interleukin-22 （IL-22） in the pathogenesis of pityriasis rosea （PR）． Methods Peripheral venous blood samples were obtained from 48 patients with PR in acute stage and recovery stage and from 20 healthy human controls. Real-time fluorescence-based quantitative PCR was performed to measure the mRNA expression of IL-22 in peripheral blood mononuclear cells （PBMCs）, and enzyme-linked immunosorbent assay （ELISA） to determine the level of IL-22 in sera. Results The relative expression level of IL-22 mRNA in the patients with PR in recovery stage was significantly higher than that in the healthy controls （2.89 ± 0.91 vs. 1.91 ± 0.60, t = 3.84, P < 0.05）, but lower than that in the patients in acute stage （2.89 ± 0.91 vs. 4.15 ± 1.36, t = 3.61, P < 0.05）. Similarly, the level of serum IL-22 was significantly increased in the patients with PR in recovery stage compared with the healthy controls （（21.44 ± 7.33） vs. （13.22 ± 4.26） ng/L, t = 4.49, P < 0.05）, and increased in the patients with PR in acute stage compared with those in recovery stage （（36.87 ± 10.63） vs. （21.44 ± 7.33） ng/L, t = 5.01, P < 0.05）. The level of serum IL-22 was positively correlated with the stage of PR （r = 0.883, P < 0.05）. Conclusions IL-22 is overexpressed in PBMCs and sera of patients with PR, implying that IL-22 is involved in the pathogenesis of PR．

Key words: Pityriasis rosea, Interleukin-22, Pathogenesis

孙怡王振华. 白细胞介素22在玫瑰糠疹患者外周血单一核细胞及血清中的表达[J]. 中华皮肤科杂志, 2014,47(10):746-747. doi:

zhenhua wang. Expressions of interleukin-22 in sera and peripheral blood mononuclear cells of patients with pityriasis rosea[J]. Chinese Journal of Dermatology, 2014, 47(10): 746-747.doi:

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