中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (8): 563-565.

• 论著 • 上一篇    下一篇

抗幽门螺杆菌抗体阳性慢性荨麻疹患者血清对人皮肤肥大细胞释放组胺作用的研究

康尔恂1,陈宏泉2,闫丽萍3,李杰1,韩春玉3,夏秀娟3   

  1. 1. 烟台毓璜顶医院
    2. 青岛大学附属医院
    3. 青岛大学医学院附属烟台毓璜顶医院
  • 收稿日期:2013-11-25 修回日期:2014-03-02 发布日期:2014-08-01
  • 通讯作者: 康尔恂 E-mail:kangerxun2000@163.com
  • 基金资助:
    山东省科技发展计划

Effect of sera from chronic urticaria patients with anti-Helicobacter pylori antibodies on the release of histamine by human mast cells

  • Received:2013-11-25 Revised:2014-03-02 Published:2014-08-01

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摘要： 【摘要】 目的 探讨抗幽门螺杆菌（Hp）抗体与慢性荨麻疹（CU）发病的关系。 方法 选择13C呼吸实验阳性、抗Hp抗体阳性的CU患者为实验组，健康人为对照组。用CU患者血清孵育人皮肤肥大细胞原代细胞株（HMC），检测血清是否可刺激HMC细胞释放组胺。用抗Hp抗体（IgG）刺激HMC细胞，检测其是否具有活化细胞释放组胺的作用。检测CU患者血清中是否也含有高亲和力受体（FcεRI）抗体或抗IgE抗体。分别用抗FcεRI抗体、抗IgE抗体刺激HMC细胞，检测其活化HMC细胞释放组胺作用。 结果 CU患者组血清活化HMC细胞释放组胺作用（3.13 ± 0.93）高于健康对照组（2.92 ± 0.75，t = 2.39，P < 0.05），4例CU患者血清中同时存在抗FcεRI抗体、3例存在抗IgE抗体。分别用这两种抗体阳性的CU患者血清孵育HMC细胞，释放组胺量均高于健康对照组（t值分别为4.82、6.34，均P < 0.01），但用只含有抗Hp抗体CU患者血清孵育HMC细胞无释放组胺作用（与健康对照组比较，t = 1.74，P > 0.05）。抗Hp抗体（IgG）无激活HMC细胞释放组胺作用（与阴性对照血清比较，t = 1.95，P > 0.05），抗FcεRI抗体、抗IgE抗体均可激活HMC细胞释放组胺（与阴性对照血清比较，t值分别为3.72、3.02，均P < 0.01）。 结论 抗Hp抗体对CU无致病作用，血清中同时存在抗FcεRI抗体或抗IgE抗体可导致部分Hp感染者同时患有CU。

关键词: 荨麻疹, 幽门螺杆菌, 抗体, 肥大细胞

Abstract: Kang Erxun*, Chen Hongquan, Yan Liping, Li Jie, Han Chunyu, Xia Xiujuan. *Department of Dermatology, Yantai Yuhuangding Hospital Affiliated to Medical College of Qingdao University, Yantai 264000, Shandong, China Corresponding author: Kang Erxun, Email: kangerxun2000@163.com 【Abstract】 Objective To investigate the relationship between anti-Helicobacter pylori （Hp） antibodies and development of chronic urticaria （CU）. Methods Fifty CU patients with positive 13C-urea breath test and anti-Hp antibodies, as well as 50 healthy human controls were recruited in this study. Serum samples were collected from all the subjects. The samples from the patients were subjected to tests for anti-high affinity IgE receptor （anti-FcεRI） and -IgE antibodies. Human mast cells （HMCs） were classified into several parts to be incubated with the sera of patients with CU, the sera of healthy controls with anti-IgE and -FcεRI antibodies respectively for 20 minutes. Those incubated with the sera of healthy controls without these antibodies served as the control. Subsequently, the levels of histamine released by HMCs were measured by enzyme-linked immunosorbent assay （ELISA）. Results The sera of CU patients showed a stronger ability to activate HMCs to release histamine than those of healthy controls （ （3.13 ± 0.93） μg/L vs （2.92 ± 0.75） μg/L, t = 2.39, P < 0.05）. Anti-FcεRI antibodies were detected in 4 patients, and anti-IgE antibodies in 3 patients. A significant increase was observed in the levels of histamine released by HMCs incubated with anti-FcεRI antibody-positive and anti-IgE antibody-positive patient-derived sera （t = 4.82, 6.34, respectively, both P < 0.01）, but not in those incubated with patient-derived sera only positive for anti-Hp antibodies （t = 1.74, P > 0.05） compared with those incubated with healthy control-derived sera. In comparison with the antibody-free healthy control-derived sera, those with anti-Hp IgG antibodies showed no significant effect on the release of histamines by HMCs （t = 1.95, P > 0.05）, whereas those with anti-FcεRI antibodies and anti-IgE antibodies exhibited an obvious promoting effect （t = 3.72, 3.02, respectively, both P < 0.01）. Conclusions The anti-Hp antibodies appears to have no role in the pathogenesis of CU, but the presence of anti-FcεRI and anti-IgE antibodies may contribute to the initiation of CU in patients with Hp infection.

Key words: Urticaria, Helicobacter pylori, Antibody, Mast cells

中图分类号: 

  • R751

引用本文

康尔恂 陈宏泉 闫丽萍 李杰 韩春玉 夏秀娟. 抗幽门螺杆菌抗体阳性慢性荨麻疹患者血清对人皮肤肥大细胞释放组胺作用的研究[J]. 中华皮肤科杂志, 2014,47(8):563-565. doi: